Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea

被引:63
作者
Park, YK [1 ]
Shin, S
Ryu, S
Cho, SN
Koh, WJ
Kwon, OJ
Shim, YS
Lew, WJ
Bai, GH
机构
[1] Korean Inst Tuberculosis, Seoul 137900, South Korea
[2] Brigham & Womens Hosp, Div Social Med & Hlth Inequalities, Boston, MA 02120 USA
[3] Yonsei Univ, Coll Med, Seoul 120752, South Korea
[4] Sungkyunkwan Univ Med, Samsung Med Ctr, Seoul 135710, South Korea
[5] Seoul Natl Univ Hosp, Dept Internal Med, Seoul 110744, South Korea
关键词
ahpC gene; Beijing family; inhA gene; katG gene; Mycobacterium tuberculosis; rpoB gene;
D O I
10.1016/j.mimet.2005.03.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The frequency of resistance genotypes among Beijing and non-Beijing strains was compared using a reverse blot hybridization assay to detect mutations within genes associated with rifampicin (rpoB) and isoniazid (katG, inhA, and ahpC) resistance. Of the 743 Mycobacterium tuberculosis isolates, 569 (77%) belonged to Beijing family. The proportion of Beijing strains was significantly higher among MDR-TB isolates than among drug-susceptible strains (82% vs. 72%, p <0.01). Genotype analysis of the rpoB gene revealed significantly lower rates of the Ser531Leu mutation rate among Beijing vs. non-Beijing MDR-TB strains (41 % vs. 66%, p < 0.005). While the mutation for Ser315Thr in the katG gene was more common among Beijing vs. non-Beijing family strains (65% vs. 50%, p<0.01), the mutation rate of promoter region of the inhA gene was lower among Beijing strains compared with non-Beijing strains (14% vs. 25%, p<0.05). Reverse hybridization successfully detected over 80% of isoniazid-resistant strains and over 92% of rifampicin-resistant strains among Korean isolates. Significant differences in mutation rates in the rpoB, katG, and inhA genes between Beijing strains and non-Beijing strains could explain discrepancies in mutation rates of genotypes in different countries. Reverse hybridization was useful for rapid detection of isoniazid and rifampicin resistant strains. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:165 / 172
页数:8
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