Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

被引：461

作者：

Schwartz, SA ^{[1
]}

Reyzer, ML ^{[1
]}

Caprioli, RM ^{[1
]}

机构：

[1] Vanderbilt Univ, Mass Spectrometry Res Ctr, Nashville, TN 37232 USA

来源：

JOURNAL OF MASS SPECTROMETRY | 2003年 / 38卷 / 07期

关键词：

sample preparation; MALDI-MS; tissue analysis; imaging MS; proteins;

D O I：

10.1002/jms.505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Practical guidelines for the preparation of tissue sections for direct analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：699 / 708

页数：10

共 20 条

[1] Mass spectrometry in proteomics [J].

Aebersold, R ;

Goodlett, DR .

CHEMICAL REVIEWS, 2001, 101 (02) :269-295

[2] Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS [J].

Caprioli, RM ;

Farmer, TB ;

Gile, J .

ANALYTICAL CHEMISTRY, 1997, 69 (23) :4751-4760

[3] Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry [J].

Chaurand, P ;

Stoeckli, M ;

Caprioli, RM .

ANALYTICAL CHEMISTRY, 1999, 71 (23) :5263-5270

[4]

CHAURAND P, 2003, P 51 ASMS C MASS SPE

[5] Direct mass spectrometric peptide profiling and sequencing of nervous tissues to identify peptides involved in male copulatory behavior in Lymnaea stagnalis [J].

Dreisewerd, K ;

Kingston, R ;

Geraerts, WPM ;

Li, KW .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 1997, 169 :291-299

[6]

Garden RW, 1996, J MASS SPECTROM, V31, P1126, DOI 10.1002/(SICI)1096-9888(199610)31:10<1126::AID-JMS403>3.0.CO

[7]

2-7

[8]

Jungblut P, 1997, MASS SPECTROM REV, V16, P145, DOI 10.1002/(SICI)1098-2787(1997)16:3<145::AID-MAS2>3.0.CO

[9]

2-H

[10] MATRIX-ASSISTED ULTRAVIOLET-LASER DESORPTION OF NONVOLATILE COMPOUNDS [J].

KARAS, M ;

BACHMANN, D ;

BAHR, U ;

HILLENKAMP, F .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 78 :53-68

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