In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly-L-lysine-treated titanium-6-aluminium-4-vanadium

被引：24

作者：

Galli, Daniela ^{[1
]}

Benedetti, Laura ^{[2
]}

Bongio, Matilde ^{[2
]}

Maliardi, Valentina ^{[2
]}

Silvani, Giulia ^{[1
]}

Ceccarelli, Gabriele ^{[1
]}

Ronzoni, Flavio ^{[1
]}

Conte, Silvio ^{[2
]}

Benazzo, Francesco ^{[3
]}

Graziano, Antonio ^{[4
]}

Papaccio, Gianpaolo ^{[4
]}

Sampaolesi, Maurilio ^{[1
,2
,5
]}

De Angelis, Maria Gabriella Cusella ^{[1
,2
]}

机构：

[1] Univ Pavia, Interdept Ctr Tissue Engn, I-27100 Pavia, Italy

[2] Univ Pavia, Dept Expt Med, Human Anat Unit, I-27100 Pavia, Italy

[3] Univ Pavia, Orthopaed Clin, IRCCS Policlin San Matteo, I-27100 Pavia, Italy

[4] Secondo Ateneo Napoli, Dipartimento Discipline Odontostomatol Ortodont &, Naples, Italy

[5] Katholieke Univ Leuven, Interdept Stem Cell Inst, Louvain, Belgium

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2011年 / 97A卷 / 02期

关键词：

Ti6Al4V; poly-L-lysine; osteoblastic differentiation; FAK; OSTEOGENIC DIFFERENTIATION; ADHESION; ORGANOAPATITE; VITRONECTIN;

D O I：

10.1002/jbm.a.32996

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through beta 1-INTEGRIN, because the presence of beta 1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 97A: 118-126, 2011.

引用

页码：118 / 126

页数：9

共 34 条

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