A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology

被引:9
作者
Corrèges, P
Bugnard, E
Millerin, C
Masiero, A
Andrivet, JP
Bloc, A
Dunant, Y
机构
[1] CMU, Dept Pharmacol, CH-1211 Geneva 4, Switzerland
[2] Ctr Interuniv Rech & Enseignement, NEUROPHARMA, F-74166 Archamps, France
[3] CERN, Div ECP, CH-1211 Geneva 23, Switzerland
关键词
electrophysiology; Peltier effect; temperature control; synaptic transmission; calcium channel;
D O I
10.1016/S0165-0270(98)00079-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8 degrees C/min) and with a good accuracy (+/- 0.5 degrees C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm(2) Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of +/- 0.1 degrees C in a range of 0-40 degrees C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the Torpedo nerve-electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:177 / 184
页数:8
相关论文
共 20 条
[1]  
Barnard R. D., 1972, THERMOELECTRICITY ME
[2]   TIME-DEPENDENT REVERSAL OF LONG-TERM POTENTIATION BY BRIEF COOLING SHOCKS IN RAT HIPPOCAMPAL SLICES [J].
BITTAR, P ;
MULLER, D .
BRAIN RESEARCH, 1993, 620 (02) :181-188
[3]   A NOVEL EXPERIMENTAL CHAMBER FOR SINGLE-CELL VOLTAGE-CLAMP AND PATCH-CLAMP APPLICATIONS WITH LOW ELECTRICAL NOISE AND EXCELLENT TEMPERATURE AND FLOW-CONTROL [J].
CANNELL, MB ;
LEDERER, WJ .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1986, 406 (05) :536-539
[4]   Disorganisation of quantal acetylcholine release by zinc at the Torpedo nerve-electroplate junction [J].
Correges, P ;
Dunant, Y .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1996, 432 (05) :859-866
[5]   VERSATILE TEMPERATURE CONTROLLED TISSUE BATH FOR STUDIES OF ISOLATED CELLS USING AN INVERTED MICROSCOPE [J].
DATYNER, NB ;
GINTANT, GA ;
COHEN, IS .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1985, 403 (03) :318-323
[6]   GRADED OR ALL-OR-NOTHING RELEASE OF TRANSMITTER QUANTA BY LOCAL DEPOLARIZATIONS OF NERVE-TERMINALS ON CRAYFISH MUSCLE [J].
DUDEL, J .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1983, 398 (02) :155-164
[7]   QUANTAL RELEASE OF ACETYLCHOLINE EVOKED BY FOCAL DEPOLARIZATION AT THE TORPEDO NERVE ELECTROPLAQUE JUNCTION [J].
DUNANT, Y ;
MULLER, D .
JOURNAL OF PHYSIOLOGY-LONDON, 1986, 379 :461-+
[8]   ACETYLCHOLINE-RELEASE EVOKED BY SINGLE OR A FEW NERVE IMPULSES IN THE ELECTRIC ORGAN OF TORPEDO [J].
DUNANT, Y ;
EDER, L ;
SERVETIADISHIRT, L .
JOURNAL OF PHYSIOLOGY-LONDON, 1980, 298 (JAN) :185-203
[9]   CALCIUM CURRENTS OF NEUROBLASTOMA X-GLIOMA HYBRID-CELLS AFTER CULTIVATION WITH DIBUTYRYL-CYCLIC-AMP AND NICKEL [J].
ECKERT, R ;
HESCHELER, J ;
KRAUTWURST, D ;
SCHULTZ, G ;
TRAUTWEIN, W .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1990, 417 (03) :329-335
[10]   A CHAMBER FOR ELECTROPHYSIOLOGICAL RECORDING FROM CULTURED NEURONS ALLOWING PERFUSION AND TEMPERATURE CONTROL [J].
FORSYTHE, ID ;
COATES, RT .
JOURNAL OF NEUROSCIENCE METHODS, 1988, 25 (01) :19-27