Identification of animal species in ground meat mixtures by multivariate analysis of isoelectric focusing protein profiles

被引：54

作者：

Skarpeid, HJ ^{[1
]}

Kvaal, K ^{[1
]}

Hildrum, KI ^{[1
]}

机构：

[1] Norwegian Food Res Inst, MATFORSK, N-1430 Aas, Norway

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 18期

关键词：

chemometrics; multivariate calibration; isoelectric focusing; animal species;

D O I：

10.1002/elps.1150191811

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present work investigates the possibility of constructing a multivariate calibration model to predict the composition of ground meat with respect to animal species, based on intensity profiles from isoelectric focusing of water-soluble proteins in mixtures of ground meat. Samples containing various amounts of beef, pork and turkey meat were analyzed by isoelectric focusing in immobilized pH-gradients. The gels were photographed and the images transferred to a digital format. The digital images were processed in order to reduce background and optimize signal strength. Resulting gel profiles were analyzed by multivariate re,regression allowing the determination of sample composition with prediction errors close to 10%.

引用

页码：3103 / 3109

页数：7

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