Simple and rapid liquid chromatographic-turbo ion spray mass spectrometric determination of topiramate in human plasma

被引:40
作者
Contin, M [1 ]
Riva, R [1 ]
Albani, F [1 ]
Baruzzi, A [1 ]
机构
[1] Univ Bologna, Inst Neurol, Lab Clin Neuropharmacol, I-40123 Bologna, Italy
来源
JOURNAL OF CHROMATOGRAPHY B | 2001年 / 761卷 / 01期
关键词
topiramate;
D O I
10.1016/S0378-4347(01)00302-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We present a simple and fast method for the determination of the novel antiepileptic drug topiramate in human plasma by high-performance liquid chromatography coupled with turbo ion spray mass spectrometry. Plasma sample pre-treatment was based on simple deproteinization by acetonitrile. Liquid chromatographic analysis was carried out on a reversed-phase column (C-18, 125 x 4 mm I.D., 5 mum) using acetonitrile-ammonium acetate buffer, pH 6.3 as the mobile phase, at a flow-rate of 0.8 ml/min. Retention time for topiramate was 2.1 min. The detector was a single quadrupole mass spectrometer coupled to a turbo ion spray ion source and a heated nebulizer probe, operating in the positive ion mode. Ion source temperature was off; voltage was +5800 V, nebulizer and curtain gas flow-rates were 6 and 10 ml/min, respectively. Calibration curves for topiramate were linear over the range 1 to 20 mug/ml. Absolute recovery ranged between 92 and 95%. Intra- and inter-assay precision was <4%. The present procedure, omitting extraction and drying steps, is faster and simpler than the previously reported analytical methods for topiramate and was demonstrated to possess adequate sensitivity for routine therapeutic drug monitoring in plasma from patients with epilepsy. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:133 / 137
页数:5
相关论文
共 17 条
[1]  
Ben-Menachem Elinor, 1995, P1063
[2]  
Chen S, 1999, RAPID COMMUN MASS SP, V13, P1980, DOI 10.1002/(SICI)1097-0231(19991030)13:20<1980::AID-RCM741>3.0.CO
[3]  
2-C
[4]  
Chen S, 2001, RAPID COMMUN MASS SP, V15, P159, DOI 10.1002/1097-0231(20010130)15:2<159::AID-RCM210>3.0.CO
[5]  
2-W
[6]   Drug therapy - New antiepileptic drugs [J].
Dichter, MA ;
Brodie, MJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1996, 334 (24) :1583-1590
[7]   Therapeutic monitoring of topiramate: Evaluation of the saturable distribution between erythrocytes and plasma of whole blood using an optimized high-pressure liquid chromatography method [J].
Gidal, BE ;
Lensmeyer, GL .
THERAPEUTIC DRUG MONITORING, 1999, 21 (05) :567-576
[8]   AUTOMATED CAPILLARY GAS-CHROMATOGRAPHIC ASSAY USING FLAME IONIZATION DETECTION FOR THE DETERMINATION OF TOPIRAMATE IN PLASMA [J].
HOLLAND, ML ;
UETZ, JA ;
NG, KT .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 433 :276-281
[9]   Topiramate in clinical practice: first year's postlicensing experience in a specialist epilepsy clinic [J].
Kellett, MW ;
Smith, DF ;
Stockton, PA ;
Chadwick, DW .
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 1999, 66 (06) :759-763
[10]  
Masucci JA, 1998, J MASS SPECTROM, V33, P85, DOI 10.1002/(SICI)1096-9888(199801)33:1<85::AID-JMS612>3.3.CO