Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza a viruses in avian cells

被引：60

作者：

Massin, P ^{[1
]}

Rodrigues, P ^{[1
]}

Marasescu, M ^{[1
]}

van der Werf, S ^{[1
]}

Naffakh, N ^{[1
]}

机构：

[1] CNRS, URA 1966, Unite Genet Mol Virus Resp, Inst Pasteur, F-75724 Paris, France

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 21期

关键词：

D O I：

10.1128/JVI.79.21.13811-13816.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Reverse genetics techniques to rescue influenza viruses have thus far been based on the use of a human polymerase I (Poll) promoter to direct the synthesis of the eight viral RNAs. They can only be used on cells from primate origin due to the species specificity of the Poll promoter. Here we report the cloning of the chicken Poll promoter sequence and the generation of recombinant influenza virus upon transfection of bidirectional PoII/PoIII plasmids in avian cells. Potential contributions of this new reverse genetics system in the fields of influenza virus research and influenza vaccine production are discussed.

引用

页码：13811 / 13816

页数：6

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