Feeder-Free Derivation of Induced Pluripotent Stem Cells From Human Immature Dental Pulp Stem Cells

被引：61

作者：

Beltrao-Braga, Patricia C. B. ^{[1
,2
,3
,4
]}

Pignatari, Graciela C. ^{[2
,3
,4
]}

Maiorka, Paulo C. ^{[2
,3
]}

Oliveira, Nelio A. J. ^{[5
]}

Lizier, Nelson F. ^{[4
,6
]}

Wenceslau, Cristiane V. ^{[2
,3
,4
]}

Miglino, Maria A. ^{[2
,3
,4
]}

Muotri, Alysson R. ^{[5
]}

Kerkis, Irina ^{[4
,6
]}

机构：

[1] Univ Sao Paulo, Sch Arts Sci & Humanities, Dept Obstet, BR-03828000 Sao Paulo, Brazil

[2] Univ Sao Paulo, Fac Vet Med, Dept Surg, BR-03828000 Sao Paulo, Brazil

[3] Univ Sao Paulo, Dept Pathol, BR-03828000 Sao Paulo, Brazil

[4] Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ribeirao Preto, Brazil

[5] Univ Calif San Diego, Sch Med, Dept Pediat,Stem Cell Program, Rady Childrens Hosp San Diego,Dept Cell & Mol Med, La Jolla, CA 92093 USA

[6] Butantan Inst, Genet Lab, Sao Paulo, Brazil

来源：

CELL TRANSPLANTATION | 2011年 / 20卷 / 11-12期

基金：

美国国家卫生研究院; 巴西圣保罗研究基金会;

关键词：

Induced pluripotent stem cells (iPSCs); Pediatric diseases; Human immature dental pulp stem cells (hIDPSCs); Reprogramming; HUMAN FIBROBLASTS; IN-VITRO; GENERATION; TRANSPLANTATION; INDUCTION; TERATOMAS;

D O I：

10.3727/096368911X566235

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Induced pluripotent stem cells (iPSCs) can be created by forcing expression of certain genes in fibroblasts or other somatic cell types, reversing them to a pluripotent state similar to that of embryonic stem cells (ESC). Here, we used human immature dental pulp stem cells (hIDPSCs) as an alternative source for creating iPSC. hIDPSCs can be easily isolated from accessible tissue of young and adult patients. hIDPSCs possess a fibroblast-like morphology, retaining characteristics of adult multipotent stem cells. Reprogramming of hIDPSCs was fast, producing primary hIDPSC-iPSC colonies even under feeder-free conditions. hIDPSCs acquired ESC-like morphology, expressed pluripotent markers, possessed stable, normal karyotypes, and demonstrated the ability to differentiated in vitro and in vivo. Our data demonstrate that hIDPSCs-iPSCs offer an advantageous cell system for future cell therapy and basic studies, particularly as a model for pediatric developmental disorders.

引用

页码：1707 / 1719

页数：13

共 37 条

[1] Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes [J].