HIV-1 A-rich RNA loop mimics the tRNA anticodon structure

被引:60
作者
Puglisi, EV [1 ]
Puglisi, JD [1 ]
机构
[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/4141
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interaction of HIV-I genomic RNA and human tRNA(3)(Lys) initiates viral reverse transcription. An adenosine-rich (A-rich) loop in HIV RNA mediates complex formation between tRNA and viral RNA. We have determined the structure of an A-rich loop oligonucleotide using nuclear magnetic resonance spectroscopy. The loop structure is stabilized by a noncanonical G-A pair and a U-turn motif, which leads to stacking of the conserved adenosines. The structure has similarity to the tRNA anticodon structure, and suggests possible mechanisms for its role in initiation of reverse transcription.
引用
收藏
页码:1033 / 1036
页数:4
相关论文
共 30 条
[1]  
Arts EJ, 1998, PROG NUCLEIC ACID RE, V58, P339
[2]   HIV-1 REVERSE-TRANSCRIPTASE SPECIFICALLY INTERACTS WITH THE ANTICODON DOMAIN OF ITS COGNATE PRIMER TRANSFER-RNA [J].
BARAT, C ;
LULLIEN, V ;
SCHATZ, O ;
KEITH, G ;
NUGEYRE, MT ;
GRUNINGERLEITCH, F ;
BARRESINOUSSI, F ;
LEGRICE, SFJ ;
DARLIX, JL .
EMBO JOURNAL, 1989, 8 (11) :3279-3285
[3]  
Batey RT, 1995, METHOD ENZYMOL, V261, P300
[4]   alpha helix-RNA major groove recognition in an HIV-1 Rev peptide RRE RNA complex [J].
Battiste, JL ;
Mao, HY ;
Rao, NS ;
Tan, RY ;
Muhandiram, DR ;
Kay, LE ;
Frankel, AD ;
Williamson, JR .
SCIENCE, 1996, 273 (5281) :1547-1551
[5]   Structure of the HIV-1 nucleocapsid protein bound to the SL3 Ψ-RNA recognition element [J].
De Guzman, RN ;
Wu, ZR ;
Stalling, CC ;
Pappalardo, L ;
Borer, PN ;
Summers, MF .
SCIENCE, 1998, 279 (5349) :384-388
[6]   HIV-1 regulatory/accessory genes: Keys to unraveling viral and host cell biology [J].
Emerman, M ;
Malim, MH .
SCIENCE, 1998, 280 (5371) :1880-1884
[7]   STRUCTURAL REQUIREMENTS FOR THE BINDING OF TRNA(3)(LYS) TO REVERSE-TRANSCRIPTASE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].
ESSINK, BBO ;
DAS, AT ;
BERKHOUT, B .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (40) :23867-23874
[8]   Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA [J].
Fourmy, D ;
Yoshizawa, S ;
Puglisi, JD .
JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (02) :333-345
[9]  
Gate JH, 1996, SCIENCE, V273, P1696, DOI 10.1126/science.273.5282.1696
[10]   ON LOOPFOLDING IN NUCLEIC-ACID HAIRPIN-TYPE STRUCTURES .29. [J].
HAASNOOT, CAG ;
HILBERS, CW ;
VANDERMAREL, GA ;
VANBOOM, JH ;
SINGH, UC ;
PATTABIRAMAN, N ;
KOLLMAN, PA .
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1986, 3 (05) :843-+