Dynamics of the COPII coat with GTP and stable analogues

被引：232

作者：

Antonny, B

Madden, D

Hamamoto, S

Orci, L

Schekman, R ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Univ Geneva, Dept Morphol, Ctr Med Univ, CH-1211 Geneva 4, Switzerland

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 06期

关键词：

D O I：

10.1038/35078500

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed an assay to monitor the assembly of the COPII coat onto liposomes in real time. We show that with Sar1pGTP bound to liposomes, a single round of assembly and disassembly of the COPII coat lasts a few seconds. The two large COPII complexes Sec23/24p and Sec13/31p bind almost instantaneously (in less than 1 s) to Sar1pGTP-doped liposomes. This binding is followed by a fast (less than 10 s) disassembly due to a 10-fold acceleration of the GTPase-activating protein activity of Sec23/24p by the Sec13/31p complex. Experiments with the phosphate analogue BeFx suggest that Sec23/24p provides residues directly involved in GTP hydrolysis on Sar1p.

引用

页码：531 / 537

页数：7

共 30 条

[1] N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange
Antonny, B
BeraudDufour, S
Chardin, P
Chabre, M
[J]. BIOCHEMISTRY, 1997, 36 (15) : 4675 - 4684
[2] The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly
Aridor, M
Fish, KN
Bannykh, S
Weissman, J
Roberts, TH
Lippincott-Schwartz, J
Balch, WE
[J]. JOURNAL OF CELL BIOLOGY, 2001, 152 (01) : 213 - 229
[3] Cargo selection by the COPII budding machinery during export from the ER
Aridor, M
Weissman, J
Bannykh, S
Nuoffer, C
Balch, WE
[J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (01) : 61 - 70
[4] BARLOWE C, 1993, J BIOL CHEM, V268, P873
[5] COPII - A MEMBRANE COAT FORMED BY SEC PROTEINS THAT DRIVE VESICLE BUDDING FROM THE ENDOPLASMIC-RETICULUM
BARLOWE, C
ORCI, L
YEUNG, T
HOSOBUCHI, M
HAMAMOTO, S
SALAMA, N
REXACH, MF
RAVAZZOLA, M
AMHERDT, M
SCHEKMAN, R
[J]. CELL, 1994, 77 (06) : 895 - 907
[6] SEC12 ENCODES A GUANINE-NUCLEOTIDE-EXCHANGE FACTOR ESSENTIAL FOR TRANSPORT VESICLE BUDDING FROM THE ER
BARLOWE, C
SCHEKMAN, R
[J]. NATURE, 1993, 365 (6444) : 347 - 349
[7] Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors
Bremser, M
Nickel, W
Schweikert, M
Ravazzola, M
Amherdt, M
Hughes, CA
Söllner, TH
Rothman, JE
Wieland, FT
[J]. CELL, 1999, 96 (04) : 495 - 506
[8] ALUMINOFLUORIDE AND BERYLLOFLUORIDE COMPLEXES - NEW PHOSPHATE ANALOGS IN ENZYMOLOGY
CHABRE, M
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (01) : 6 - 10
[9] THE ARF1 GTPASE-ACTIVATING PROTEIN - ZINC-FINGER MOTIF AND GOLGI-COMPLEX LOCALIZATION
CUKIERMAN, E
HUBER, I
ROTMAN, M
CASSEL, D
[J]. SCIENCE, 1995, 270 (5244) : 1999 - 2002
[10] TRYPTOPHAN-W207 IN TRANSDUCIN T-ALPHA IS THE FLUORESCENCE SENSOR OF THE G-PROTEIN ACTIVATION SWITCH AND IS INVOLVED IN THE EFFECTOR-BINDING
FAUROBERT, E
OTTOBRUC, A
CHARDIN, P
CHABRE, M
[J]. EMBO JOURNAL, 1993, 12 (11) : 4191 - 4198

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