Long period grating based biosensor for the detection of Escherichia coli bacteria

被引：158

作者：

Tripathi, Saurabh Mani ^{[1
]}

Bock, Wojtek J. ^{[1
]}

Mikulic, Predrag ^{[1
]}

Chinnappan, Raja ^{[2
]}

Ng, Andy ^{[2
]}

Tolba, Mona ^{[2
]}

Zourob, Mohammed ^{[2
]}

机构：

[1] Univ Quebec Outaouais, Ctr Rech Photon, Dept Informat Ingn, Gatineau, PQ J8Y 3G5, Canada

[2] Inst Natl Rech Sci Energie Mat & Telecommun, Varennes, PQ J3X 1S2, Canada

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 35卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Bacteriophages; Long period fiber grating; Spectral interrogation; Pathogen; Covalent binding; SURFACE-PLASMON RESONANCE; PHAGE;

D O I：

10.1016/j.bios.2012.03.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper we report a stable, label-free, bacteriophage-based detection of Escherichia coli (E. coli) using ultra sensitive long-period fiber gratings (LPFGs). Bacteriophage T4 was covalently immobilized on optical fiber surface and the E. coli binding was investigated using the highly accurate spectral interrogation mechanism. In contrast to the widely used surface plasmon resonance (SPR) based sensors, no moving part or metal deposition is required in our sensor, making the present sensor extremely accurate, very compact and cost effective. We demonstrated that our detection mechanism is capable of reliable detection of E. colt concentrations as low as 10(3) cfu/ml with an experimental accuracy greater than 99%. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：308 / 312

页数：5

共 20 条

[1] Biosensors as innovative tools for the detection of food borne pathogens [J].

Arora, Pooja ;

Sindhu, Annu ;

Dilbaghi, Neeraj ;

Chaudhury, Ashok .

BIOSENSORS & BIOELECTRONICS, 2011, 28 (01) :1-12

[2] Detection of insulin-antibody binding on a solid surface using imaging ellipsometry [J].

Bae, YM ;

Oh, BK ;

Lee, W ;

Lee, WH ;

Choi, JW .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (04) :895-902

[3] RNA biosensor for the rapid detection of viable Escherichia coli in drinking water [J].

Baeumner, AJ ;

Cohen, RN ;

Miksic, V ;

Min, JH .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (04) :405-413

[4] Lytic phage as a specific and selective probe for detection of Staphylococcus aureus -: A surface plasmon resonance spectroscopic study [J].

Balasubramanian, Shankar ;

Sorokulova, Iryna B. ;

Vodyanoy, Vitaly J. ;

Simonian, Aleksandr L. .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (06) :948-955

[5]

Chandran SP, 2008, CURR SCI INDIA, V95, P1327

[6] Fiber grating spectra [J].

Erdogan, T .

JOURNAL OF LIGHTWAVE TECHNOLOGY, 1997, 15 (08) :1277-1294

[7]

Homola J, 2006, SPRINGER SER CHEM SE, V4, P1, DOI 10.1007/b100321

[8] Optical waveguide sensor for on-line monitoring of bacteria [J].

Horváth, R ;

Pedersen, HC ;

Skivesen, N ;

Selmeczi, D ;

Larsen, NB .

OPTICS LETTERS, 2003, 28 (14) :1233-1235

[9]

Kashyap R, 2010, FIBER BRAGG GRATINGS, 2ND EDITION, P1

[10] Highly sensitive phage-based biosensor for the detection of β-galactosidase [J].

Nanduri, Viswaprakash ;

Balasubramanian, Shankar ;

Sista, Srinivas ;

Vodyanoy, Vitaly J. ;

Simonian, Aleksandr L. .

ANALYTICA CHIMICA ACTA, 2007, 589 (02) :166-172

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