A discontinuous method for catalase determination at 'near physiological' concentrations of H2O2 and its application to the study of H2O2 fluxes within cells

被引：111

作者：

Ou, PM ^{[1
]}

Wolff, SP ^{[1
]}

机构：

[1] UCL, SCH MED,RAYNE INST,DEPT MED, DIV CLIN PHARMACOL & TOXICOL, LONDON WC1E 7JJ, ENGLAND

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 31卷 / 1-2期

关键词：

catalase; hydrogen peroxide; measurement;

D O I：

10.1016/0165-022X(95)00039-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a discontinuous method for the measurement of catalase using the Ferrous Oxidation in Xylenol orange (FOX) assay. Samples containing catalase are incubated with H2O2 for varying time intervals prior to rapid mixing of aliquots of the incubation mixtures with FOX reagent, which measures residual H2O2. Absorbance is then read at 560 nm after 30-min incubation at room temperature. Decay of H2O2 is proportional to catalase activity in the original sample. An adaptation of the method involves exposure of intact cells to H2O2-generating systems in the presence of aminotriazole. The extent of catalase inactivation allows estimates of total H2O2 accumulation within the cell.

引用

页码：59 / 67

页数：9

共 12 条

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