Spectrophotometric determination of vitamin C with iron(II)-4-(2-pyridylazo)resorcinol complex

被引：35

作者：

Arya, SP ^{[1
]}

Mahajan, M ^{[1
]}

Jain, P ^{[1
]}

机构：

[1] Kurukshetra Univ, Dept Chem, Kurukshetra 136119, Haryana, India

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 427卷 / 02期

关键词：

vitamin C determination; 4-(2-pyridylazo)resorcinol; potassium citrate; extraction; n-butanol;

D O I：

10.1016/S0003-2670(00)01092-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A spectrophotometric method has been developed for the determination of vitamin C in a variety of samples. The procedure is based on the reduction of iron(III) to iron(II) and complexation of iron(II) with 4-(2-pyridylazo)resorcinol, followed by its extraction into n-butanol. The absorbance is measured at 710 nm. Beer's law is observed up to 5.5 mug ml(-1) ascorbic acid. Interference effects of various substances including sugars, vitamins, amino acids, inorganic cations and anions and some organic substances have been studied. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 251

页数：7

共 19 条

[1] Al-Zamil I. Z., 1986, Oriental Journal of Chemistry, V2, P6
[2] Arya SP, 1998, CHEM ANAL-WARSAW, V43, P231
[3] Nitroso-R salt as a sensitive spectrophotometric reagent for the determination of vitamin C
Arya, SP
Mahajan, M
[J]. ANALYTICAL LETTERS, 1997, 30 (14) : 2541 - 2553
[4] Arya SP, 1998, ANN CHIM-ROME, V88, P429
[5] ARYA SP, 1997, P NATL ACAD SCI INDI, V67, P39
[6] A FACILE AND SENSITIVE SPECTROPHOTOMETRIC DETERMINATION OF ASCORBIC-ACID
BESADA, A
[J]. TALANTA, 1987, 34 (08) : 731 - 732
[7] RAPID MANUAL METHOD FOR ROUTINE ASSAY OF ASCORBIC-ACID IN SERUM AND PLASMA
DAY, BR
WILLIAMS, DR
MARSH, CA
[J]. CLINICAL BIOCHEMISTRY, 1979, 12 (01) : 22 - 26
[8] Sensitive spectrophotometric determination of ascorbic acid in fruit juices and pharmaceutical formulations using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP)
Ferreira, SLC
Bandeira, MLSF
Lemos, VA
dosSantos, HC
Costa, ACS
deJesus, DS
[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1997, 357 (08): : 1174 - 1178
[9] GOPALAN C, 1997, NUTR VALUE INDIAN FO, P66
[10] HARRIS LJ, 1955, VITAMINS THEORY PRAC, P130

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