DNA binding and nuclease activity of a one-dimensional heterometallic nitrosyl complex

被引：55

作者：

Selim, Md ^{[1
]}

Chowdhury, Sujoy Roy ^{[1
]}

Mukherjea, Kalyan K. ^{[1
]}

机构：

[1] Jadavpur Univ, Dept Chem, Kolkata 700032, W Bengal, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2007年 / 41卷 / 05期

关键词：

one-dimensional complex; pUC19 supercoiled DNA; nuclease activity; spectrophotometry; spectrofluorimetry; viscometry; gel-electrophoresis;

D O I：

10.1016/j.ijbiomac.2007.07.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The interaction of a structurally characterized Sr-Fe nitrosyl complex with DNA has been studied by UV-vis and fluorescence spectroscopy, viscometric, and gel electrophoresis techniques. From the absorption titration studies the intrinsic binding constant of the complex with DNA was calculated to be 1.6 x 10(4) M-1. Fluorimetric studies indicate that the complex compete with EB in binding to DNA. The complex shows nuclease activity on pUC19 supercoiled DNA in presence of H2O2. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：579 / 583

页数：5

共 52 条

[1]

QUENCHING OF DNA-ETHIDIUM FLUORESCENCE BY AMSACRINE AND OTHER ANTITUMOR AGENTS - A POSSIBLE ELECTRON-TRANSFER EFFECT [J].