High-performance liquid chromatography with postcolumn derivatization for simultaneous determination of organic peroxides and hydrogen peroxide

A reversed-phase HPLC procedure with enzyme mediated postcolumn derivatization and fluorescence detection has been developed for the simultaneous analysis of organic peroxides such as 1-((1-hydroperoxycyclohexyl)-dioxy)-cyclohexanol (HDC), t-butyl hydroperoxide (BHP) and cumene hydroperoxide (CHP), as well as hydrogen peroxide. The detection limits are 6.0 ng for H2O2, 0.15 mu g for HDC, 3.7 mu g for BHP and 7.0 mu g for CHP respectively. Detection limits may be enhanced by preadsorption of the organic peroxides on a mixture of XAD-4 and XAD-8 resins with methanol elution. The method was used to detect a metastable peroxide intermediate from the ozonation of hexadeca-9-enoic acid. (C) 1998 Elsevier Science B.V. All rights reserved.