Identification of the reaction products of (2′-5′)oligoadenylate synthetase in the marine sponge

被引:30
作者
Kuusksalu, A
Subbi, J
Pehk, T
Reintamm, T
Müller, WEG
Kelve, M
机构
[1] Inst Chem Phys & Biophys, Mol Genet Lab, EE-0026 Tallinn, Estonia
[2] Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-6500 Mainz, Germany
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 257卷 / 02期
关键词
(2 '-5 ')oligoadenylate; Geodia cydonium; matrix-assisted laser-desorption-ionization MS; NMR;
D O I
10.1046/j.1432-1327.1998.2570420.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previously we reported on the presence of a high (2'-5')oligoadenylate synthetase activity in the marine sponge Geodia cydonium [Kuusksalu, A., Pihlak, A., Muller, W. E. G. & Kelve. M. (1995) Eur. J. Biochem. 232, 351-357]. The presence of (2'-5')oligoadenylates [(2'-5')A] in crude sponge extract was shown by radioimmunoassay and by their HPLC comigration with authentic (2'-5')A oligomers. Tn addition, the sponge (2'-5')oligoadenylates displayed biological activity, as determined by inhibition studies of protein biosynthesis in rabbit reticulocyte lysate. In the present study individual (2'-5')oligoadenylates synthesized by sponge enzyme were separated by HPLC. The exact composition of every oligonucleotide peak eluted was determined by matrix-assisted laser-desorption-ionization mass spectrometry (MALDI-MS) analysis. The 2'-5' phosphodiester bond in oligoadenylates was verified by NMR analysis. Based on the high concentration of (2'-5')A oligomers in G. cydonium and their similarity with those found in mammals we propose that the (2'-5')A system is involved in a cytokine-mediated pathway and/or in a protection system against viruses, present in the marine environment.
引用
收藏
页码:420 / 426
页数:7
相关论文
共 57 条
[1]   Oligonucleotide sequence and composition determined by matrix-assisted laser desorption ionization [J].
Bentzley, CM ;
Johnston, MV ;
Larsen, BS ;
Gutteridge, S .
ANALYTICAL CHEMISTRY, 1996, 68 (13) :2141-2146
[2]  
BOOGAART EVD, 1994, EUR J BIOCHEM, V221, P759
[3]   Type IV collagen in sponges, the missing link in basement membrane ubiquity [J].
Boute, N ;
Exposito, JY ;
BouryEsnault, N ;
Vacelet, J ;
Noro, N ;
Miyazaki, K ;
Yoshizato, K ;
Garrone, R .
BIOLOGY OF THE CELL, 1996, 88 (1-2) :37-44
[4]  
Brown R E, 1981, Methods Enzymol, V79, P208
[5]   DISTRIBUTION OF THE PPP(A2'P)NA BINDING-PROTEIN AND INTERFERON-RELATED ENZYMES IN ANIMALS, PLANTS, AND LOWER ORGANISMS [J].
CAYLEY, PJ ;
WHITE, RF ;
ANTONIW, JF ;
WALESBY, NJ ;
KERR, IM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 108 (03) :1243-1250
[6]  
CHEBATH J, 1987, J BIOL CHEM, V262, P3852
[7]   CONFORMATIONAL-ANALYSIS OF THE NUCLEOTIDES A2'-5'A, A2'-5'A2'-5'A AND A2'-5'U FROM NUCLEAR MAGNETIC-RESONANCE AND CIRCULAR-DICHROISM STUDIES [J].
DOORNBOS, J ;
DENHARTOG, JAJ ;
VANBOOM, JH ;
ALTONA, C .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 116 (02) :403-412
[8]   SEPARATION OF DNA RESTRICTION FRAGMENTS BY ION-PAIR CHROMATOGRAPHY [J].
ERIKSSON, S ;
GLAD, G ;
PERNEMALM, PA ;
WESTMAN, E .
JOURNAL OF CHROMATOGRAPHY, 1986, 359 :265-274
[9]   A sequencing method for RNA oligonucleotides based on mass spectrometry [J].
Faulstich, K ;
Worner, K ;
Brill, H ;
Engels, JW .
ANALYTICAL CHEMISTRY, 1997, 69 (21) :4349-4353
[10]   THE ANALYSIS OF MOCK DNA-SEQUENCING REACTIONS USING MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY [J].
FITZGERALD, MC ;
ZHU, L ;
SMITH, LM .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (10) :895-897