Functionalized temperature-sensitive copolymer for tissue engineering of articular cartilage and meniscus

被引：18

作者：

Chen, Jyh-Ping ^{[1
]}

Cheng, Tai-Hong ^{[1
]}

机构：

[1] Chang Gung Univ, Dept Chem & Mat Engn, Tao Yuan 333, Taiwan

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2008年 / 313卷

关键词：

temperature-sensitive copolymer; hydrogel; tissue engineering; N-isopropylacrylamide;

D O I：

10.1016/j.colsurfa.2007.04.130

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 [物理化学]; 081704 [应用化学];

摘要：

Thermo-sensitive chitosan-g-poly(N-isopropylacrylamide) (CPN) and hyaluronic acid-g-CPN (HA-CPN) copolymer were fabricated by forming amide bond linkages between carboxyl-ended poly(N-isopropylacrylamide), chitosan, and HA. Aqueous polymer solutions showed reversibly soluble-insoluble phase transition characteristics, which is free-flowing at room temperature and turns into a rigid gel structure at 37 degrees C. SEM observation demonstrated an inter-connected microporous structure with pore-size ranging from 20 to 50 mu m. Chondrocytes and meniscus cells attached and proliferated well when cultured on the copolymer hydrogel. The cells maintained high viability and showed distinct morphology. The aggregated chondrocytes on the thermo-reversible hydrogel surface can be easily detached and recovered by simply lowering the temperature. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：254 / 259

页数：6

共 9 条

[1]

Internalization of the hyaluronan receptor CD44 by chondrocytes [J].