Recombinant viral capsids as an efficient vehicle of oligonucleotide delivery into cells

被引：31

作者：

Cooper, A ^{[1
]}

Shaul, Y ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 327卷 / 04期

关键词：

HBV capsid; core particle; oligonucleotide delivery; encaspidation;

D O I：

10.1016/j.bbrc.2004.12.118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Delivery of oligonucleotides (ON) into cells is a technical challenge. In this study, we utilized the capsid of the hepatitis B virus (HBV) to meet this goal. A single and short open reading frame of the virus programs efficient capsid production in bacteria. We show that these capsids can encapsulate ON in vitro and then mediate their delivery into cells with extreme efficiency. This process is cell type non-specific, rendering the recombinant HBV capsid a potentially valuable vehicle for ON delivery into a wide range of cultured cells. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1094 / 1099

页数：6

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