Chlorophyll a fluorescence: A tool for the investigation of toxic effects in the photosynthetic apparatus

被引：66

作者：

Brack, W ^{[1
]}

Frank, H ^{[1
]}

机构：

[1] Univ Bayreuth, Dept Environm Chem & Ecotoxicol, D-95447 Bayreuth, Germany

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 1998年 / 40卷 / 1-2期

关键词：

D O I：

10.1006/eesa.1997.1639

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The effects of environmental pollutants, such as triazines, urea herbicides, phenols, nitro aromatic compounds, aldehydes, hydrogen sulfide, volatile halogenated aliphatic and aromatic compounds, and alkylbenzenes, the photosynthesis of green algae were investigated by using detailed analysis of chlorophyll alpha fluorescence. Application of quenching analysis by means of a pulse amplitude-modulated fluorometer allows the discrimination of different modes of action. Blocks of the electron-transport chain, the action of uncouplers, and fluorescence quenching by nitroaormatic compounds could be distinguished. Hydrogen sulfide and short-chain aldehydes seem to react in the same may, probably by reaction with components of the electron-transport chain. Nonreactive volatile compounds affect chlorophyll fluorescence in a strictly hydrophobicity-dependent manner. The quenching analysis suggests an inhibition of utilization of proton-motive force for ATP synthesis. Detailed analysis of fluorescence patterns may be of great help for identification of toxic pollutants and their sources. (C) 1998 Academic Press.

引用

页码：34 / 41

页数：8

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