Application of SSB-like protein from Thermus aquaticus in multiplex PCR of human Y-STR markers identification

被引：16

作者：

Olszewski, M

Rebala, K

Szczerkowska, Z

Kur, J

机构：

[1] Gdansk Tech Univ, Dept Microbiol, PL-80952 Gdansk, Poland

[2] Med Univ Gdansk, Dept Forens Med, PL-80210 Gdansk, Poland

来源：

MOLECULAR AND CELLULAR PROBES | 2005年 / 19卷 / 03期

关键词：

multiplex PCR; thermophilic bacteria; thermostability; Y-STR;

D O I：

10.1016/j.mcp.2004.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we demonstrate the application of SSB-like protein from Thermus aquaticus (TaqSSB) in Multiplex PCR of human Y-STR markers identification. The use of thermostable TaqSSB prevents or reduces primer dimmer formation, one of the particular problems known to cause inhibition of primer hybridization to the template and reduction of a number of primers available for annealing in PCR reaction. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：203 / 205

页数：3

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