Intermolecular interactions between the SH3 domain and the proline-rich TH region of Bruton's tyrosine kinase

被引：34

作者：

Hansson, H

Okoh, MP

Smith, CIE

Vihinen, M

Härd, T ^{[1
]}

机构：

[1] Royal Inst Technol, Novum, Ctr Struct Biochem, Dept Biotechnol, S-14157 Huddinge, Sweden

[2] Univ Tampere, Inst Med Technol, FIN-33101 Tampere, Finland

[3] Univ Helsinki, Dept Biosci, Div Biochem, FIN-00014 Helsinki, Finland

[4] Karolinska Inst, Novum, Dept Biosci, S-14157 Huddinge, Sweden

[5] Huddinge Univ Hosp, Karolinska Inst, Clin Res Ctr, S-14186 Huddinge, Sweden

[6] Tampere Univ Hosp, FIN-33520 Tampere, Finland

来源：

FEBS LETTERS | 2001年 / 489卷 / 01期

关键词：

Bruton's tyrosine kinase; Src homology 3; dimerization; nuclear magnetic resonance; gel permeation chromatography; signal transduction;

D O I：

10.1016/S0014-5793(00)02438-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SH3 domain of Bruton's tyrosine kinase (Btk) is preceded by the Tec homology (TH) region containing proline-rich sequences. We have studied a protein fragment containing both the Btk SH3 domain and the proline-rich sequences of the TH region (PRR-SH3). Intermolecular NMR cross-relaxation measurements, gel permeation chromatography profiles, titrations with proline-rich peptides, and N-15 NMR relaxation measurements are all consistent with a monomer-dimer equilibrium with a dissociation constant on the order of 60 muM. The intermolecular interactions do, at least in part, involve proline-rich sequences in the TH region. This behavior of Btk PRR-SH3 may have implications for the functional action of Btk. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：67 / 70

页数：4

共 23 条

[1] Regulatory intramolecular association in a tyrosine kinase of the Tec family [J].