Magnetic characterisation of rat muscle tissues after subcutaneous iron dextran injection

被引:37
作者
Lázaro, FJ
Abadía, AR
Romero, MS
Gutiérrez, L
Lázaro, J
Morales, MP
机构
[1] Ctr Politecn Super, Dept Ciencia & Tecnol Mat & Fluidos, Zaragoza 50018, Spain
[2] Univ Zaragoza, CSIC, Inst Ciencia Mat Aragon, Zaragoza 50018, Spain
[3] Univ Zaragoza, Dept Fisiol & Farmacol, E-50013 Zaragoza, Spain
[4] Univ Zaragoza, Dept Med & Psiquiat, E-50009 Zaragoza, Spain
[5] Univ Zaragoza, Dept Anat Patol, E-50009 Zaragoza, Spain
[6] CSIC, Inst Ciencia Mat, E-28049 Madrid, Spain
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2005年 / 1740卷 / 03期
关键词
iron dextran; AC susceptibility; superparamagnetism; iron speciation; iron metabolism; pharmacokinetics;
D O I
10.1016/j.bbadis.2004.11.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ex vivo freeze-dried rat muscle tissues, collected at different times t after a single dose of subcutaneously injected iron dextran, have been magnetically characterised. The AC susceptibility of the tissues shows an overall superparamagnetic behaviour and the dependence on t of, especially, the out-of-phase component is remarkably systematic despite the fact that each tissue originates in a different rat individual. The experiments show that the akaganete (beta-FeOOH) nanoparticles contained in the injected drug are progressively degraded in the living tissue and, at times of the order of 1 month and for all the analysed rat individuals, converge to a magnetically well-defined species with much narrower magnetic activation energy distribution than iron dextran. Thorough transmission electron microscopy experiments of the same tissues indicate the presence of oxyhydroxide particles, whose size decreases for increasing t in agreement with the interpretation of the magnetic susceptibility. The conclusions drawn from the magnetic study do well correspond to the properties of the whole tissue since no biochemical extraction work has been done. The AC susceptibility appears to be a valuable and complementary tool in pharmacological studies of iron-containing drugs. (c) 2004 Elsevier B.V All rights reserved.
引用
收藏
页码:434 / 445
页数:12
相关论文
共 28 条
[1]   Low-frequency low-field magnetic susceptibility of ferritin and hemosiderin [J].
Allen, PD ;
St Pierre, TG ;
Chua-anusorn, W ;
Ström, V ;
Rao, KV .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2000, 1500 (02) :186-196
[2]  
Andrews S C, 1988, Biol Met, V1, P33, DOI 10.1007/BF01128015
[3]  
[Anonymous], 2002, PROTEINS IRON METABO, DOI DOI 10.1201/B14262
[4]   Magnetic investigations of human mesencephalic neuromelanin [J].
Bolzoni, F ;
Giraudo, S ;
Lopiano, L ;
Bergamasco, B ;
Fasano, M ;
Crippa, PR .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 2002, 1586 (02) :210-218
[5]   Noninvasive methods for quantitative assessment of transfusional iron overload in sickle cell disease [J].
Brittenham, GM ;
Sheth, S ;
Allen, CJ ;
Farrell, DE .
SEMINARS IN HEMATOLOGY, 2001, 38 (01) :37-56
[6]   Trace elements in bovine muscle:: an ongoing project for a new certified reference material [J].
Caroli, S ;
Spagnoli, M ;
Forte, G ;
Alessandrelli, M ;
Cresti, R ;
D'Ilio, S ;
Pauwels, J ;
Kramer, GN ;
Záray, G .
MICROCHEMICAL JOURNAL, 2000, 67 (1-3) :235-243
[7]   RAPID INDUCTION OF HEPATIC-FIBROSIS IN THE GERBIL AFTER THE PARENTERAL ADMINISTRATION OF IRON-DEXTRAN COMPLEX [J].
CARTHEW, P ;
EDWARDS, RE ;
SMITH, AG ;
DORMAN, B ;
FRANCIS, JE .
HEPATOLOGY, 1991, 13 (03) :534-539
[8]   The effect of prolonged iron loading on the chemical form of iron oxide deposits in rat liver and spleen [J].
Chua-anusorn, W ;
Webb, J ;
Macey, DJ ;
Hall, PDM ;
St Pierre, TG .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1999, 1454 (02) :191-200
[9]   Magnetic properties of human liver and brain ferritin [J].
Dubiel, SM ;
Zablotna-Rypien, B ;
Mackey, JB ;
Williams, JM .
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1999, 28 (03) :263-267
[10]  
FISCHER R, 1998, MAGNETISM MED