Matrix solid-phase dispersion technique for the determination of moxidectin in bovine tissues

An HPLC method has been developed for the determination of moxidectin in bovine tissues, The extraction and clean-up procedure is based on the matrix solid-phase dispersion technique. Control and moxidectin-fortified bovine tissue samples (0.25 g) are blended with octadecyl (C-18 end-capped) packing material. A column made from the C-18-bovine tissue blend is mashed with hexane (2 ml); when all the hexane has eluted, an Alumina-B SPE cartridge is attached below the C-18-tissue column and, after washing, moxidectin is eluted with methanol (6 ml). Moxidectin is derivatized and determined by HPLC with fluorescence detection, The recovery from fortified samples was greater than 80% in the concentration range 1-100 ng g(-1) of tissue, This method permits the determination of moxidectin at levels as low as 1 ng g(-1) (1 ppb).