Hypertonic saline activation of P38 MAPK primes the PMN respiratory burst

被引:27
作者
Ciesla, DJ
Moore, EE
Biffl, WL
Gonzalez, RJ
Moore, HB
Silliman, CC
机构
[1] Denver Hlth Med Ctr, Dept Surg, Denver, CO 80204 USA
[2] Univ Colorado, Ctr Hlth Sci, Dept Pediat, Bonfils Blood Ctr, Denver, CO 80204 USA
来源
SHOCK | 2001年 / 16卷 / 04期
关键词
inflammation; neutrophil; cytotoxicity; signaling;
D O I
10.1097/00024382-200116040-00009
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
investigation of hypertonic saline (HTS) modulation of neutrophils (PMN) cytotoxic responses has generated seemingly contradictory results. Clinically relevant levels of HTS attenuate receptor-mediated p38 MAPK signaling, whereas higher levels activate p38 MAPK. Concurrently, HTS exerts a dose-dependent attenuation of the PMN respiratory burst, most notably at concentrations where p38 MAPK is activated. We hypothesized that HTS-mediated p38 MAPK activation augments the PMN respiratory burst on return to normotonicity. We found that although clinically relevant levels of HTS (Na+ greater than or equal to 200 mM) did not activate p38 MAPK, higher concentrations (Na+ greater than or equal to 300 mM) resulted in activation comparable with that after PAF stimulation. Transient stimulation with high levels of HTS primed the PMN respiratory burst in response to fMLP and PMA. This effect was attenuated by pretreatment with SB 203580, a p38 MAPK specific inhibitor. We conclude that severe osmotic shock primes the respiratory burst via p38 MAPK signaling, further supporting the role of this signaling cascade in PMN priming.
引用
收藏
页码:285 / 289
页数:5
相关论文
共 42 条
[1]   Hypertonic saline resuscitation diminishes lung injury by suppressing neutrophil activation after hemorrhagic shock [J].
Angle, N ;
Hoyt, DB ;
Coimbra, R ;
Liu, F ;
Herdon-Remelius, C ;
Loomis, W ;
Junger, WG .
SHOCK, 1998, 9 (03) :164-170
[2]   AN OSMOSENSING SIGNAL TRANSDUCTION PATHWAY IN YEAST [J].
BREWSTER, JL ;
DEVALOIR, T ;
DWYER, ND ;
WINTER, E ;
GUSTIN, MC .
SCIENCE, 1993, 259 (5102) :1760-1763
[3]   THE PRODUCTION OF CYTOKINES BY POLYMORPHONUCLEAR NEUTROPHILS [J].
CASSATELLA, MA .
IMMUNOLOGY TODAY, 1995, 16 (01) :21-26
[4]   Hypertonic saline inhibits neutrophil (PMN) priming via attenuation of p38 MAPK signaling [J].
Ciesla, DJ ;
Moore, EE ;
Gonzalez, RJ ;
Biffl, WL ;
Silliman, CC .
SHOCK, 2000, 14 (03) :265-269
[5]   Hypertonic saline attenuation of polymorphonuclear neutrophil cytotoxicity: Timing is everything [J].
Ciesla, DJ ;
Moore, EE ;
Zallen, G ;
Biffl, WL ;
Silliman, CC .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 2000, 48 (03) :388-395
[6]  
CIESLA DJ, 1999, SURG FORUM, V50, P189
[7]   HOW MAP KINASES ARE REGULATED [J].
COBB, MH ;
GOLDSMITH, EJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (25) :14843-14846
[8]   Hypertonic saline dextran attenuates leukocyte accumulation in the liver after hemorrhagic: Shock and resuscitation [J].
Corso, CO ;
Okamoto, S ;
Rüttinger, D ;
Messmer, K .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1999, 46 (03) :417-423
[9]   SB-203580 IS A SPECIFIC INHIBITOR OF A MAP KINASE HOMOLOG WHICH IS STIMULATED BY CELLULAR STRESSES AND INTERLEUKIN-1 [J].
CUENDA, A ;
ROUSE, J ;
DOZA, YN ;
MEIER, R ;
COHEN, P ;
GALLAGHER, TF ;
YOUNG, PR ;
LEE, JC .
FEBS LETTERS, 1995, 364 (02) :229-233
[10]  
Detmers PA, 1998, J IMMUNOL, V161, P1921