Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

被引：22

作者：

Mishra, Baijayantimala ^{[1
]}

Sharma, Mirnalini ^{[1
]}

Pujhari, Sujit Kumar ^{[1
]}

Ratho, Radha Kanta ^{[1
]}

Gopal, D. V. R. Sai ^{[2
]}

Kumar, C. V. M. Naresh ^{[2
]}

Sarangi, Gita ^{[3
]}

Chayani, Nirupama ^{[3
]}

Varma, Subhash C. ^{[4
]}

机构：

[1] Post Grad Inst Med Educ & Res, Dept Virol, Chandigarh, India

[2] Sri Venkateshwara Univ Tirupati, Dept Virol, Tirupati, Andhra Pradesh, India

[3] SCB Med Coll, Dept Microbiol, Cuttack, Orissa, India

[4] Post Grad Inst Med Educ & Res, Dept Internal Med, Chandigarh, India

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 71卷 / 02期

关键词：

Multiplex RT-PCR; Dengue; Chikungunya; Diagnosis; Serotyping; DISEASE; CLUSTAL;

D O I：

10.1016/j.diagmicrobio.2011.06.020

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：118 / 125

页数：8

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