Biotransformation of 4-chloro-2-nitrophenol into 5-chloro-2-methylbenzoxazole by a marine Bacillus sp strain MW-1

被引:33
作者
Arora, Pankaj Kumar [1 ]
Jain, Rakesh Kumar [1 ]
机构
[1] CSIR, Inst Microbial Technol, Sect 39A, Chandigarh 160036, India
关键词
4-chloro-2-nitrophenol; 4-chloro-2-acetaminophenol; 5-chloro-2-methylbenzoxazole; Biotransformation; Detoxification; PSEUDOMONAS-PUTIDA; DEGRADATION;
D O I
10.1007/s10532-011-9512-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
Decolourization, detoxification and biotransformation of 4-chloro-2-nitrophenol (4C2NP) by Bacillus sp. strain MW-1 were studied. This strain decolorized 4C2NP only in the presence of an additional carbon source. On the basis of thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), 4-chloro-2-aminophenol, 4-chloro-2-acetaminophenol and 5-chloro-2-methylbenzoxazole were identified as metabolites. Resting cells depleted 4C2NP with stoichiometric formation of 5-chloro-2-methyl benzoxazole. This is the first report of the formation of 5-chloro-2-methylbenzoxazole from 4C2NP by any bacterial strain.
引用
收藏
页码:325 / 331
页数:7
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