The crystals of a mannose-specific jacalin-related lectin from Morus nigra are merohedrally twinned

被引：4

作者：

Rabijns, A

Verboven, C

de Armas, HN

Van Damme, EJM

Peumans, WJ

De Ranter, CJ

机构：

[1] KU Leuven, Fac Farmaceut Wetenschappen, Lab Analyt Chem & Med Fysicochem, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Fac Landbouwkundige & Toegepaste Biol Wetenschapp, Lab Fytopathol & Plantenbescherming, B-3001 Louvain, Belgium

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901002104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MornigaM, a lectin from Morus nigra, belongs to the mannose-binding subgroup of the family of jacalin-related plant lectins. It was crystallized in the P6(5) space group, with unit-cell parameters a = b = 110.74, c = 159.28 Angstrom. The partially merohedrally twinned crystals could be detwinned and a subsequent molecular-replacement solution could be found using the coordinates of jacalin. Preliminary analysis clearly shows the tetrameric assembly of this protein. Furthermore, data from MornigaM crystals soaked in a mannose solution were collected.

引用

收藏

页码：609 / 611

页数：3

相关论文

共 12 条

[1] Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins [J].

Bourne, Y ;

Zamboni, V ;

Barre, A ;

Peumans, WJ ;

Van Damme, EJM ;

Rougé, P .

STRUCTURE, 1999, 7 (12) :1473-1482

[2]

BRUENGER AT, 1987, SCIENCE, V235, P458

[3] SPARSE-MATRIX SAMPLING - A SCREENING METHOD FOR CRYSTALLIZATION OF PROTEINS [J].

JANCARIK, J ;

KIM, SH .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1991, 24 :409-411

[4] Structure of the complex of Maclura pomifera agglutinin and the T-antigen disaccharide, Galβ1,3GalNAc [J].

Lee, X ;

Thompson, A ;

Zhang, ZM ;

Ton-that, H ;

Biesterfeldt, J ;

Ogata, C ;

Xu, LL ;

Johnston, RAZ ;

Young, NM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (11) :6312-6318

[5] Processing of X-ray diffraction data collected in oscillation mode [J].

Otwinowski, Z ;

Minor, W .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326

[6] Higher plants developed structurally different motifs to recognize foreign glycans [J].

Peumans, WJ ;

Barre, A ;

Hao, Q ;

Rougé, P ;

Van Damme, EJM .

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 2000, 12 (64) :83-101

[7] The galactose-binding and mannose-binding jacalin-related lectins are located in different sub-cellular compartments [J].

Peumans, WJ ;

Hause, B ;

Van Damme, EJM .

FEBS LETTERS, 2000, 477 (03) :186-192

[8] Isolation of a novel plant lectin with an unusual specificity from Calystegia sepium [J].

Peumans, WJ ;

Winter, HC ;

Bemer, V ;

VanLeuven, F ;

Goldstein, IJ ;

TruffaBachi, P ;

VanDamme, EJM .

GLYCOCONJUGATE JOURNAL, 1997, 14 (02) :259-265

[9] A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold [J].

Sankaranarayanan, R ;

Sekar, K ;

Banerjee, R ;

Sharma, V ;

Surolia, A ;

Vijayan, M .

NATURE STRUCTURAL BIOLOGY, 1996, 3 (07) :596-603

[10] PHASE ANNEALING IN SHELX-90 - DIRECT METHODS FOR LARGER STRUCTURES [J].

SHELDRICK, GM .

ACTA CRYSTALLOGRAPHICA SECTION A, 1990, 46 :467-473