Detection of Babesia caballi and Babesia equi in Dermacentor nuttalli adult ticks

被引:70
作者
Battsetseg, B
Xuan, XN
Ikadai, H
Bautista, JLR
Byambaa, B
Boldbaatar, D
Battur, B
Battsetseg, G
Batsukh, Z
Igarashi, I
Nagasawa, H
Mikami, T
Fujisaki, K
机构
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
[3] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Vet Parasitol, Towada, Aomori 0348628, Japan
[4] Vet Res Inst, Dept Ecoparasitol & Protozool, Zaisan 210153, Ulaanbaatar, Mongolia
关键词
Babesia caballi; Babesia equi; Dermacentor nuttalli; Mongolia; polymerase chain reaction;
D O I
10.1016/S0020-7519(01)00120-5
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Ticks play an important role in human and veterinary medicine particularly due to their ability to transmit protozoan pathogens. In this study we have demonstrated that polymerase chain reaction (PCR) and nested PCR methods: enabled detection of Babesia caballi and Babesia equi in field isolates of Dermacentor nuttalli adult ticks from Mongolia. Primers specific for 218 bp fragment merozoite antigen 1 (EMA-1) gene of B. equi successfully amplified products from all samples of D. nuttalli adult ticks while primers for the 430 bp fragment product from BC48 gene of B. caballi amplified products from seven of the 54 samples. Using PCR and nested PCR methods we have found mixed infections with B. equi and B. caballi in the tick vector. The amplified DNA fragment from D. nuttalli ticks was inserted into the EcoRV site of pBluescript SK and sequenced. The sequence of the 430 bp fragment was completely identical to the nucleotide sequence of the USDA strain of B. caballi. These results suggest that D. nuttalli may play an important role as a vector of both B. caballi and B. equi and also may be important in maintaining endemicity of equine piroplasmosis in Mongolia. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
引用
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页码:384 / 386
页数:3
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