Optimal activation of isopsoralen to prevent amplicon carryover

被引:7
作者
Fahle, GA [1 ]
Gill, VJ [1 ]
Fischer, SH [1 ]
机构
[1] NIH, Warren G Magnuson Clin Ctr, Microbiol Serv, Dept Clin Pathol, Bethesda, MD 20892 USA
关键词
D O I
10.1128/JCM.37.1.261-262.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5 degrees C. Our data suggest that use of the HRI-300 reaction chamber at 5 degrees C results in a statistically significantly higher degree of amplicon neutralization.
引用
收藏
页码:261 / 262
页数:2
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