Cosolute effect on crystallization of two dinucleotide complexes of bovine seminal ribonuclease from concentrated salt solutions

被引：9

作者：

Sica, F ^{[1
]}

Adinolfi, S ^{[1
]}

Vitagliano, L ^{[1
]}

Zagari, A ^{[1
]}

Capasso, S ^{[1
]}

Mazzarella, L ^{[1
]}

机构：

[1] CNR,CTR STUDIO BIOCRISTALLOGRAFIA,I-80134 NAPLES,ITALY

来源：

JOURNAL OF CRYSTAL GROWTH | 1996年 / 168卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(96)00354-5

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Two complexes of bovine seminal ribonuclease with dinucleotides, uridylyl(2'-5')adenosine (UpA) and 2'-deoxycytidylyl(3'-5')-2'-deoxyadenosine (d(CpA)), were crystallized under unusual conditions involving a liquid-liquid phase separation. This phenomenon was induced by adding small aliquots of organic cosolutes to highly concentrated ammonium sulfate solutions. The liquid-liquid interface acts as a source of nucleation centers for growth of large crystals. Among the cosolutes tested in these salt-mediated crystallizations, 2-methyl-2,4-pentanediol was found to be the most efficient.

引用

页码：192 / 197

页数：6

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