Isolation of Burkholderia cepacia complex genomovars from waters

被引：40

作者：

Vermis, K

Brachkova, M

Vandamme, P

Nelis, H ^{[1
]}

机构：

[1] Univ Ghent, Lab Pharmaceut Microbiol, B-9000 Ghent, Belgium

[2] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2003年 / 26卷 / 04期

关键词：

Burkholderia cepacia complex; genomovar; water; isolation; B; cepacia; multivorans; cenocepacia; vietnamiensis; anthina;

D O I：

10.1078/072320203770865909

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this study was to develop a selective enrichment broth as an aid for the isolation of Burkholderia cepacia complex (Bcc) bacteria from water. To allow growth of all nine genomovars, mixtures of two carbon sources had to be used, i.e. L-arabinose/D-cellobiose or L-arabinose/L-threonine. Selectivity was provided by polymyxin B and 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390). Following enrichment, Bcc bacteria were isolated on a diagnostic O/F agar supplemented with gentamicin. A preliminary bio-diversity study on 28 surface waters yielded five different genomovars, i.e. B. cepacia (genomovar I), B. multivorans, B. cenocepacia, B. vietnamiensis and B. anthina. Drinking waters did not contain Bcc bacteria. However, the genomovar pattern from a given sample varied with the enrichment broth used.

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页码：595 / 600

页数：6

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