Glutamine synthetase induction by glucocorticoids is preserved in skeletal muscle of aged rats

被引：12

作者：

MeynialDenis, D ^{[1
]}

Mignon, M ^{[1
]}

Miri, A ^{[1
]}

Imbert, J ^{[1
]}

Aurousseau, E ^{[1
]}

Taillandier, D ^{[1
]}

Attaix, D ^{[1
]}

Arnal, M ^{[1
]}

Grizard, J ^{[1
]}

机构：

[1] CTR RECH NUTR HUMAINE CLERMONT FERRAND, CLERMONT FERRAND, FRANCE

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1996年 / 271卷 / 06期

关键词：

soleus; tibialis anterior; heart; aging; dexamethasone;

D O I：

10.1152/ajpendo.1996.271.6.E1061

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Glutamine synthetase (GS) is a glucocorticoid-inducible enzyme that has a key role for glutamine synthesis in muscle. We hypothesized that the glucocorticoid induction of GS could be altered in aged rats, because alterations in the responsiveness of some genes to glucocorticoids were reported in aging. We compared the glucocorticoid-induced GS in fast-twitch and slow-twitch skeletal muscles (tibialis anterior and soleus, respectively) and heart from adult (age 6-8 mo) and aged (age 22 mo) female rats. All animals received dexamethasone (Dex) in their drinking water (0.77 +/- 0.10 and 0.80 +/- 0.08 mg/day per adult and aged rat, respectively) for 5 days. Dex caused an increase in both GS activity and GS mRNA in fast-twitch and slow-twitch skeletal muscles from adult and aged rats. In contrast, Dex increased GS activity in heart of adult rats, without any concomitant change in GS mRNA levels. Furthermore, Dex did not affect CTS activity in aged heart. Thus the responsiveness of GS to an excess of glucocorticoids is preserved in skeletal muscle but not in heart from aged animals.

引用

页码：E1061 / E1066

页数：6

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