Mitochondrial DNA polymorphism: its role in longevity of the Irish population

被引:147
作者
Ross, OA
McCormack, R
Curran, MD
Duguid, RA
Barnett, YA
Rea, IM
Middleton, D
机构
[1] Belfast City Hosp, No Ireland REg Histocompatibil & Immunogenet Lab, Belfast BT9 7TS, Antrim, North Ireland
[2] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland
[3] Queens Univ Belfast, Dept Geriatr Med, Belfast BT7 1NN, Antrim, North Ireland
[4] Queens Univ Belfast, Sch Biol & Biochem, Belfast BT7 1NN, Antrim, North Ireland
关键词
mitochondrial DNA; longevity; J haplogroup; polymorphism;
D O I
10.1016/S0531-5565(01)00094-8
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
The mtDNA genome has been implicated as playing a pivotal role in determining the longevity and success of the human lifespan. A PCR-RFLP methodology was used to identify polymorphic restriction enzyme sites within a 2643 bp region of the mtDNA genome and a table of genetic haplotypes for a healthy aged and a younger control cohort of patients was constructed. Forty-six different mtDNA haplotypes and 11 groups of related haplotypes were identified across the two age groups but statistical analysis failed to show any significant associations. The European J haplogroup, previously reported to be associated with longevity, was not found at an increased frequency within the Irish aged population (P = 0.36). However, the haplotypes comprising the J haplogroup could be differentiated into two distinct branches by the presence or absence of the two polymorphic restriction sites, 16389g and 16000g. The branch of haplotypes defined by 16389g displayed a significant increased frequency in the aged samples (8%) compared to the controls (1%), P = 0.015. Inversely, the branch of haplotypes defined by 16000g displayed a significant decreased frequency in the aged samples (4%) compared to the controls (13%), P = 0.011. The polymorphism (mt5178A) associated with longevity in the Japanese was not found in the Irish population, while the polymorphism (mt9055A) associated with successful ageing in the French centenarians was found at an increased frequency in the Irish aged population (9%) compared to the younger control group (5%), but failed to reach a level of statistical significance, P = 0.164, (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:1161 / 1178
页数:18
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