The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor

被引：368

作者：

Prokhortchouk, A

Hendrich, B

Jorgensen, H

Ruzov, A

Wilm, M

Georgiev, G

Bird, A

Prokhortchouk, E

机构：

[1] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[2] Inst Gene Biol, Grp Transcript Control & Oncogenesis, Moscow, Russia

[3] European Mol Biol Lab, Prot & Peptide Grp, D-69117 Heidelberg, Germany

[4] Inst Gene Biol, Dept Mol Canc Genet, Moscow, Russia

来源：

GENES & DEVELOPMENT | 2001年 / 15卷 / 13期

关键词：

DNA methylation; methy-CpG binding protein; Kaiso; transcriptional repression; POZ/BTB zinc-finger protein;

D O I：

10.1101/gad.198501

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5 ' CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. We find that Kaiso behaves as a methylation-dependent transcriptional repressor in transient transfection assays. Kaiso is a constituent of one of two methyl-CpG binding complexes originally designated as MeCP1. The data suggest that zinc-finger motifs are responsible for DNA binding, and may therefore target repression to specific methylated regions of the genome. As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.

引用

页码：1613 / 1618

页数：6

共 32 条

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