Real-time detection of 60-fs isomerization in a rhodopsin analog containing eight-membered-ring retinal

被引:61
作者
Kandori, H
Sasabe, H
Nakanishi, K
Yoshizawa, T
Mizukami, T
Shichida, Y
机构
[1] KYOTO UNIV,FAC SCI,DEPT BIOPHYS,SAKYO KU,KYOTO 60601,JAPAN
[2] INST PHYS & CHEM RES,FRONTIER RES PROGRAM,WAKO,SAITAMA 35101,JAPAN
[3] COLUMBIA UNIV,DEPT CHEM,NEW YORK,NY 10027
[4] OSAKA SANGYO UNIV,FAC ENGN,DEPT INFORMAT SYST ENGN,DAITO,OSAKA 574,JAPAN
关键词
D O I
10.1021/ja951665h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Femtosecond fluorescence spectroscopy has been used to investigate the excited-state dynamics of a rhodopsin analog having retinal modified by a flexible eight-membered ring. The fluorescence lifetime was determined to be 60 fs throughout the observed wavelengths. Combined with the previous transient absorption results (Mizukami, T.; Kandori, H.; Shichida, Y.; Chen, A.-H.; Derguini, F.; Caldwell, C. G.; Bigge, C. F.; Nakanishi, K.; Yoshizawa, T. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 4072-4076), the ultrafast process was interpreted in terms of rapid deformation around the C11-C12 double bond, i.e., isomerization, occurring as fast as vibrational motions of the chromophore. The ultrafast reaction suggests that the isomerization occurs in a coherent manner. On the basis of the present results for the modified rhodopsin, the primary processes in the native rhodopsin are discussed.
引用
收藏
页码:1002 / 1005
页数:4
相关论文
共 26 条
[1]   2-PHOTON SPECTROSCOPY OF LOCKED-11-CIS-RHODOPSIN - EVIDENCE FOR A PROTONATED SCHIFF-BASE IN A NEUTRAL PROTEIN-BINDING SITE [J].
BIRGE, RR ;
MURRAY, LP ;
PIERCE, BM ;
AKITA, H ;
BALOGHNAIR, V ;
FINDSEN, LA ;
NAKANISHI, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4117-4121
[2]   PHOTOPHYSICS AND MOLECULAR ELECTRONIC APPLICATIONS OF THE RHODOPSINS [J].
BIRGE, RR .
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1990, 41 :683-733
[3]   SYNTHESIS OF RETINALS WITH 8-MEMBERED AND 9-MEMBERED RINGS IN THE SIDE-CHAIN - MODELS FOR RHODOPSIN PHOTOBLEACHING INTERMEDIATES [J].
CALDWELL, CG ;
DERGUINI, F ;
BIGGE, CF ;
CHEN, AH ;
HU, SH ;
WANG, J ;
SASTRY, L ;
NAKANISHI, K .
JOURNAL OF ORGANIC CHEMISTRY, 1993, 58 (13) :3533-3537
[4]   FLUORESCENCE RELAXATION KINETICS FROM RHODOPSIN AND ISORHODOPSIN [J].
DOUKAS, AG ;
LU, PY ;
ALFANO, RR .
BIOPHYSICAL JOURNAL, 1981, 35 (02) :547-550
[5]  
DOUKAS AG, 1984, P NATL ACAD SCI-BIOL, V81, P4790, DOI 10.1073/pnas.81.15.4790
[6]   FEMTOSECOND TIME-RESOLVED FLUORESCENCE SPECTROSCOPY OF BACTERIORHODOPSIN - DIRECT OBSERVATION OF EXCITED-STATE DYNAMICS IN THE PRIMARY STEP OF THE PROTON PUMP CYCLE [J].
DU, M ;
FLEMING, GR .
BIOPHYSICAL CHEMISTRY, 1993, 48 (02) :101-111
[7]  
FLEMING GR, 1986, CHEM APPLICATION ULT
[8]   UNBLEACHABLE RHODOPSIN WITH AN 11-CIS-LOCKED 8-MEMBERED RING RETINAL - THE VISUAL TRANSDUCTION PROCESS [J].
HU, SH ;
FRANKLIN, PJ ;
WANG, J ;
SILVA, BER ;
DERGUINI, F ;
NAKANISHI, K .
BIOCHEMISTRY, 1994, 33 (02) :408-416
[9]   MOLECULAR MECHANISM FOR INITIAL PROCESS OF VISUAL EXCITATION .1. MODEL OF PHOTOISOMERIZATION IN RHODOPSIN AND ITS THEORETICAL BASIS BY A QUANTUM MECHANICAL CALCULATION OF ADIABATIC POTENTIAL [J].
KAKITANI, T ;
KAKITANI, H .
JOURNAL OF THE PHYSICAL SOCIETY OF JAPAN, 1975, 38 (05) :1455-1463
[10]   EXCITED-STATE DYNAMICS OF RETINAL PROTEINS AS STUDIED BY FOURIER-TRANSFORM OF OPTICAL-ABSORPTION SPECTRUM .1. DEVELOPMENT OF ANALYTICAL METHOD [J].
KAKITANI, T ;
HATANO, Y ;
SHICHIDA, Y ;
IMAMOTO, Y ;
TOKUNAGA, F ;
KAKITANI, H .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1992, 56 (06) :977-987