Increased ciliary neurotrophic factor expression in reactive astrocytes following spinal cord injury in the rat

被引：52

作者：

Lee, MY

Kim, CJ

Shin, SL

Moon, SH

Chun, MH

机构：

[1] Catholic Univ, Coll Med, Dept Anat, Seoul 137701, South Korea

[2] Catholic Univ, Coll Med, Dept Anesthesiol, Seoul 137701, South Korea

来源：

NEUROSCIENCE LETTERS | 1998年 / 255卷 / 02期

关键词：

ciliary neurotrophic factor; astrocytes; Western blotting; immunocytochemistry; spinal cord; rat;

D O I：

10.1016/S0304-3940(98)00710-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF-immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF-like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：79 / 82

页数：4

共 13 条

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