Increased expression of the transcription factor E2F1 during dopamine-evoked, caspase-3-mediated apoptosis in rat cortical neurons

被引：23

作者：

Hou, ST ^{[1
]}

Cowan, E ^{[1
]}

Dostanic, S ^{[1
]}

Rasquinha, I ^{[1
]}

Comas, T ^{[1
]}

Morley, P ^{[1
]}

MacManus, JP ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

NEUROSCIENCE LETTERS | 2001年 / 306卷 / 03期

关键词：

cortical neurons; E2F1; transcription factor; caspase-3; apoptosis;

D O I：

10.1016/S0304-3940(01)01909-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The transcription factor E2F1 mRNA and protein levels increased in rat cortical neurons in response to dopamine (DA)or 6-hydroxydopamine (OHDA)-evoked apoptosis. Increased E2F1 protein was detected in the nucleus of neurons by double fluorescent immunocytochemistry using antibodies to E2F1 and NeuN. DA and 6-OHDA induced caspase-3-mediated apoptosis of cortical neurons which was attenuated by the addition of antioxidants or caspase-3 inhibitors to the cultures. Antioxidants prevented DA-evoked neuronal apoptosis, and also attenuated the increase in E2F1 expression. These findings suggest that increased expression of the transcription factor E2F1 may serve as a death signal during DA-evoked neuronal apoptosis. (C) 2001 Published by Elsevier Science Ireland Ltd.

引用

页码：153 / 156

页数：4

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