α-Lipoic acid ameliorates impaired glucose uptake in LYRM1 overexpressing 3T3-L1 adipocytes through the IRS-1/Akt signaling pathway

被引：17

作者：

Qin, Zhen-Ying ^{[1
]}

Zhang, Min ^{[5
,6
]}

Guo, Xi-Rong ^{[5
,6
]}

Wang, Yu-Mei ^{[3
]}

Zhu, Guan-Zhong ^{[2
]}

Ni, Yu-Hui ^{[5
,6
]}

Zhao, Ya-Ping ^{[4
]}

Qiu, Jie ^{[5
,6
]}

Kou, Chun-Zhao ^{[2
]}

Qin, Rui ^{[1
]}

Cao, Xin-Guo ^{[5
,6
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Nanjing 210036, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Inst Pediat, Nanjing 210029, Jiangsu, Peoples R China

[3] Huaian Matern & Child Hlth Hosp, Dept Child Hlth, Huaian 223002, Peoples R China

[4] 82 Hosp Peoples Liberat Army, Dept Clin Lab, Huaian 223001, Peoples R China

[5] Nanjing Med Univ, Dept Pediat, Nanjing 210036, Jiangsu, Peoples R China

[6] Nanjing Med Univ, Nanjing Maternal & Child Hlth Care Hosp, Nanjing 210036, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2012年 / 44卷 / 05期

基金：

中国国家自然科学基金;

关键词：

alpha-LA; LYRM1; Mitochondrial dysfunction; Insulin resistance; Obesity; OXIDATIVE STRESS; INSULIN-RESISTANCE; MITOCHONDRIAL DYSFUNCTION; REACTIVE OXYGEN; GLUT4; TRANSLOCATION; SKELETAL-MUSCLE; OBESE-PATIENTS; STIMULATION; TRANSPORT; RECEPTOR;

D O I：

10.1007/s10863-012-9460-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

Overexpression of the Homo sapiens LYR motif containing 1 (LYRM1) causes mitochondrial dysfunction and induces insulin resistance in 3T3-L1 adipocytes. alpha-Lipoic acid (alpha-LA), a dithiol compound with antioxidant properties, improves glucose transport and utilization in 3T3-L1 adipocytes. The aim of this study was to investigate the direct effects of alpha-LA on reactive oxygen species (ROS) production and insulin sensitivity in LYRM1 overexpressing 3T3-L1 adipocytes and to explore the underlying mechanism. Pretreatment with alpha-LA significantly increased both basal and insulin-stimulated glucose uptake and insulin-stimulated GLUT4 translocation, while intracellular ROS levels in LYRM1 overexpressing 3T3-L1 adipocytes were decreased. These changes were accompanied by a marked upregulation in expression of insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt following treatment with alpha-LA. These results indicated that alpha-LA protects 3T3-L1 adipocytes from LYRM1-induced insulin resistance partially via its capacity to restore mitochondrial function and/or increase phosphorylation of IRS-1 and Akt.

引用

页码：579 / 586

页数：8

共 44 条

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