Phosphorus speciation and high-affinity transporters are influenced by humic substances

被引:54
作者
Jindo, Keiji [1 ,2 ]
Soares, Tatiane Sanches [1 ]
Pereira Peres, Lazaro Eustaquio [3 ]
Azevedo, Inga Golalvez [1 ]
Aguiar, Natalia Oliveira [1 ]
Mazzei, Pierluigi [4 ]
Spaccini, Riccardo [4 ]
Piccolo, Alessandro [4 ]
Olivares, Fabio Lopes [1 ]
Canellas, Luciano Pasqualoto [1 ]
机构
[1] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, Nucleo Desenvolvimento Insumos Biol Agr, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil
[2] Univ Tokyo, Inst Ind Sci, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan
[3] Univ Sao Paulo, ESALQ, Dept Ciencias Biol, Piracicaba, Brazil
[4] Univ Naples Federico II, CERMANU, Ctr Interdipartimentale Ric Risonanza Magnet Nucl, Portici, Italy
关键词
humic acids; phosphate transporter; vermicompost; H+-ATPASE ACTIVITY; NITRATE UPTAKE; ROOT; PHOSPHATE; GROWTH; DEFICIENCY; EXPRESSION; GENES; ACIDS; ACQUISITION;
D O I
10.1002/jpln.201500228
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Phosphorus (P) is a limiting factor for plant growth, especially in highly weathered tropical soils. Plants have several mechanisms to overcome low P availability in soil, such as humic substances, that reduce phosphate (P-i) adsorption on oxide surfaces and enhance soil P availability. However, the direct influence of humic substances on P-i transporters in root cells or the distribution of P species in leaves remains unclear. Tomato seedlings were grown in a sand-vermiculite mixture with low or high P concentrations (10 or 100 mg kg(-1) KH2PO4, respectively) and humic acids (0 or 48 mg C L-1) isolated from vermicompost. Plant responses were evaluated in the fifth week by measuring root and shoot weights and P concentration, and differential expression in the roots of the high-affinity P-i transporter genes LePT1 and LePT2. In addition, the distribution of P species in the leaves was assessed using P-31-NMR. Humic acids increased the root biomass and changed the distribution of P species in the leaves. Inorganic phosphate was the major compound in plants supplied with a high P concentration, whereas in plants supplied with a low P concentration, P-i was only identified in plants not treated with humic acids. Glycerophosphodiester and phosphorylcholine accumulated in plants treated with humic acid, indicating a modified metabolic pathway for economical P consumption at low P concentrations. High transcript accumulation of LePT2 was observed in roots treated with humic acids at both P concentrations. Our results show that humic substances are strategically involved in plant adaptation to P availability.
引用
收藏
页码:206 / 214
页数:9
相关论文
共 45 条
[1]   Identification of Phosphatin, a Drug Alleviating Phosphate Starvation Responses in Arabidopsis [J].
Arnaud, Carole ;
Clement, Mathilde ;
Thibaud, Marie-Christine ;
Javot, Helene ;
Chiarenza, Serge ;
Delannoy, Etienne ;
Revol, Julia ;
Soreau, Paul ;
Balzergue, Sandrine ;
Block, Maryse A. ;
Marechal, Eric ;
Desnos, Thierry ;
Nussaume, Laurent .
PLANT PHYSIOLOGY, 2014, 166 (03) :1479-+
[2]  
BLIGNY R, 1990, J BIOL CHEM, V265, P1319
[3]   Humic acids crossinteractions with root and organic acids [J].
Canellas, L. P. ;
Teixeira Junior, L. R. L. ;
Dobbss, L. B. ;
Silva, C. A. ;
Medici, L. O. ;
Zandonadi, D. B. ;
Facanha, A. R. .
ANNALS OF APPLIED BIOLOGY, 2008, 153 (02) :157-166
[4]   Probing the hormonal activity of fractionated molecular humic components in tomato auxin mutants [J].
Canellas, L. P. ;
Dantas, D. J. ;
Aguiar, N. O. ;
Peres, L. E. P. ;
Zsoegoen, A. ;
Olivares, F. L. ;
Dobbss, L. B. ;
Facanha, A. R. ;
Nebbioso, A. ;
Piccolo, A. .
ANNALS OF APPLIED BIOLOGY, 2011, 159 (02) :202-211
[5]   Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots [J].
Canellas, LP ;
Olivares, FL ;
Okorokova-Façanha, AL ;
Façanha, AR .
PLANT PHYSIOLOGY, 2002, 130 (04) :1951-1957
[6]   Physiological responses to humic substances as plant growth promoter [J].
Canellas L.P. ;
Olivares F.L. .
Chemical and Biological Technologies in Agriculture, 1 (1)
[7]   Genome-wide investigation and expression analysis suggest diverse roles and genetic redundancy of Pht1 family genes in response to Pi deficiency in tomato [J].
Chen, Aiqun ;
Chen, Xiao ;
Wang, Huimin ;
Liao, Dehua ;
Gu, Mian ;
Qu, Hongye ;
Sun, Shubin ;
Xu, Guohua .
BMC PLANT BIOLOGY, 2014, 14
[8]  
Chen Y, 2004, ADV AGROECOL, P103
[9]   White Lupin Cluster Root Acclimation to Phosphorus Deficiency and Root Hair Development Involve Unique Glycerophosphodiester Phosphodiesterases [J].
Cheng, Lingyun ;
Bucciarelli, Bruna ;
Liu, Junqi ;
Zinn, Kelly ;
Miller, Susan ;
Patton-Vogt, Jana ;
Allan, Deborah ;
Shen, Jianbo ;
Vance, Carroll P. .
PLANT PHYSIOLOGY, 2011, 156 (03) :1131-1148
[10]   NUTRIENT SOLUTION GROWTH OF SORGHUM AND CORN IN MINERAL-NUTRITION STUDIES [J].
CLARK, RB .
JOURNAL OF PLANT NUTRITION, 1982, 5 (08) :1039-1057