Extracellular hydrolases of strain Bacillus sp 739 and their involvement in the lysis of micromycete cell walls

被引:23
作者
Aktuganov, G. E. [1 ]
Galimzyanova, N. F. [1 ]
Melent'ev, A. I. [1 ]
Kuz'mina, L. Yu. [1 ]
机构
[1] Russian Acad Sci, Ufa Res Ctr, Inst Biol, Moscow 117901, Russia
关键词
mycolytic enzymes; Bacillus spp; chitinase; beta-1,3-glucanase; antagonism; phytopathogenic fungi;
D O I
10.1134/S0026261707040054
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The mycolytic bacterial strain Bacillus sp. 739 produces extracellular enzymes which degrade in vitro the cell walls of a number of phytopathogenic and saprophytic fungi. When Bacillus sp. 739 was cultivated with Bipolaris sorokiniana, a cereal root-rot pathogen, the fungus degradation process correlated with the levels of the beta-1,3-glucanase and protease activity. The comparative characteristic of Bacillus sp. 739 enzymatic preparations showed that efficient hydrolysis of the fungus cell walls was the result of the action of the complex of enzymes produced by the strain when grown on chitin-containing media. Among the enzymes of this complex, chitinases and beta-1,3-glucanases hydrolyzed most actively the disintegrated cell walls of B. sorokiniana. However, only beta-1,3-glucanases were able to degrade the cell walls of native fungal mycelium in the absence of other hydrolases, which is indicative of their key role in the mycolytic activity of Bacillus sp. 739.
引用
收藏
页码:413 / 420
页数:8
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