Inhibition of astrocyte TNFα expression by extracellular potassium

被引:6
作者
Chang, HY [1 ]
Hua, LWL [1 ]
Morgan, A [1 ]
Lee, SC [1 ]
机构
[1] Albert Einstein Coll Med, Dept Pathol F717, Bronx, NY 10461 USA
关键词
brain; hyperkalemia; cytokine; inflammation;
D O I
10.1016/S0006-8993(00)03098-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
TNF alpha and IL-6 are cytokines of great interest, given the numerous biological activities and the documented expression in several central nervous system (CNS) pathologies. In this report, we have examined cultures of IL-1- or IL-1/IFN gamma -activated human fetal astrocytes as a model to study mechanisms of cytokine regulation in the inflamed CNS. Since one of the major functions of astrocytes is spatial buffering of K+ ions, we examined the effect of high extracellular KCl on astrocyte cytokine expression by ribonuclease protection assay and ELISA. Results demonstrate that astrocyte TNF alpha production was potently inhibited by K+ with 44 and 89% inhibition at 25 and 55 mM K+, respectively. In contrast, astrocyte IL-6 inhibition required higher concentrations of K+ (greater than or equal to 75 mM). These results demonstrate a novel role for astrocyte potassium channel activity in modulation of glial cytokine production. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:176 / 179
页数:4
相关论文
共 18 条
[1]   ION CHANNEL EXPRESSION BY WHITE MATTER GLIA - THE TYPE-1 ASTROCYTE [J].
BARRES, BA ;
KOROSHETZ, WJ ;
CHUN, LLY ;
COREY, DP .
NEURON, 1990, 5 (04) :527-544
[2]   Cytokine actions in the central nervous system [J].
Benveniste, EN .
CYTOKINE & GROWTH FACTOR REVIEWS, 1998, 9 (3-4) :259-275
[3]  
Downen M, 1999, GLIA, V28, P114, DOI 10.1002/(SICI)1098-1136(199911)28:2<114::AID-GLIA3>3.0.CO
[4]  
2-O
[5]   EXTRACELLULAR POTASSIUM CONCENTRATION IN JUVENILE AND ADULT RAT-BRAIN CORTEX DURING ANOXIA [J].
HANSEN, AJ .
ACTA PHYSIOLOGICA SCANDINAVICA, 1977, 99 (04) :412-420
[6]  
HOPKINS SJ, 1995, TRENDS NEUROSCI, V18, P83
[7]   CATION ACTIVITIES IN REVERSIBLE ISCHEMIA OF CAT BRAIN [J].
HOSSMANN, KA ;
SAKAKI, S ;
ZIMMERMANN, V .
STROKE, 1977, 8 (01) :77-81
[8]  
Hua LWL, 2000, GLIA, V30, P74, DOI 10.1002/(SICI)1098-1136(200003)30:1<74::AID-GLIA8>3.0.CO
[9]  
2-C
[10]   Interleukin-1, nitric oxide and reactive astrocytes [J].
Lee, SC ;
Dickson, DW ;
Brosnan, CF .
BRAIN BEHAVIOR AND IMMUNITY, 1995, 9 (04) :345-354