CD81 and microglial activation in vitro:: proliferation, phagocytosis and nitric oxide production

被引：26

作者：

Dijkstra, S

Geisert, EE

Dijkstra, CD

Bär, PR

Joosten, EAJ

机构：

[1] UMC Utrecht, Dept Expt Neurol, NL-3508 GA Utrecht, Netherlands

[2] Univ Tennessee, Memphis Coll Med, Dept Anat & Neurobiol, Memphis, TN 38163 USA

[3] Free Univ Amsterdam, Fac Med, Dept Cell Biol & Immunol, NL-1081 BT Amsterdam, Netherlands

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2001年 / 114卷 / 1-2期

关键词：

microglia; tetraspanins; CD81; trauma; nervous system; spinal cord injuries;

D O I：

10.1016/S0165-5728(01)00240-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

CD81 (TAPA), a member of the tetraspanin family of proteins, is upregulated by astrocytes and microglia after traumatic injury to the rat central nervous system (CNS). To further understand the role of CD81 in the microglial response to injury, we analysed the functional effects of a CD81 antibody, AMP1, on cultured rat microglia. We found that AMP1 suppressed microglial proliferation in a dose-dependent manner. Furthermore, AMP1 stimulated myelin phagocytosis, probably by opsonizing the myelin. The phagocytosis of latex beads, as well as the production of nitric oxide, were not significantly influenced by AMP1. These data indicate that CD81 is involved in an important subset of microglial effector functions after CNS injury. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 159

页数：9

共 52 条

[1] Mechanisms of phagocytosis in macrophages [J].