Microbiological processes in the terrestrial carbon cycle: Methane cycling in peat

被引:40
作者
Edwards, C
Hales, BA
Hall, GH
McDonald, IR
Murrell, JC
Pickup, R
Ritchie, DA [1 ]
Saunders, JR
Simon, BM
Upton, M
机构
[1] Univ Liverpool, Donnan Labs, Sch Biol Sci, Liverpool L69 3BX, Merseyside, England
[2] Inst Freshwater Ecol, Ambleside LA22 0LP, Cumbria, England
[3] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
关键词
methanogens; methanotrophs; molecular probes; polymerase chain reaction; microbial diversity;
D O I
10.1016/S1352-2310(98)00107-1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Coring and cutting devices were developed to extract 30 cm cores;from pear under conditions which preserve their physical/spatial integrity, maintain anoxic conditions and allow transverse sectioning. Methane production potential, measured on 1-2 cm vertical sections, was broadly absent from the upper 5 cm, maximum in the next 10 cm and thereafter declined. Methane oxidation potential was maximum in the upper 10 cm and thereafter detected in reducing amounts. Molecular methods, based on the extraction of total DNA and its PGR amplification and hybridisation with specific phylogenetic 16S rRNA and functional gene specific primers and probes, were developed to analyse the methane producing and methane oxidising microbial communities. The functional gene sequences were based on methanogen methyl coenzyme reductase and methanotroph methane monooxygenase. Methanotrophs are located throughout the core whereas methanogens are restricted to the lower zones. The composition of the microbial communities is however complex consisting of a number of novel species not previously recognised and with the various species/sub-groups occupying different vertical zones. (C) 1948 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:3247 / 3255
页数:9
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