Neuro-glial differentiation of human bone marrow stem cells in vitro

被引:175
作者
Bossolasco, P
Cova, L
Calzarossa, C
Rimoldi, SG
Borsotti, C
Deliliers, GL
Silani, V
Soligo, D
Polli, E
机构
[1] Univ Milan, Sch Med, IRCCS, Ist Auxol Italiano,Dino Ferrari Ctr,Dept Neurol, I-20149 Milan, Italy
[2] Univ Milan, Sch Med, IRCCS, Ist Auxol Italiano,Dino Ferrari Ctr,Lab Neurosci, I-20149 Milan, Italy
[3] Fdn Matarelli, Milan, Italy
[4] Univ Milan, Osped Maggiore, Sch Med, IRCCS,Bone Marrow Transplantat Unit, Milan, Italy
关键词
mesenchymal stem cells; hematopoietic stem cells; trans-differentiation; human adult stem cells; CD90(+);
D O I
10.1016/j.expneurol.2004.12.013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Bone marrow (BM) is a rich source of stem cells and may represent a valid alternative to neural or embryonic cells in replacing autologous damaged tissues for neurodegenerative diseases. The purpose of the present study is to identify human adult BM progenitor cells capable of neuro-glial differentiation and to develop effective protocols of trans-differentiation to surmount the hematopoietic commitment in vitro. Heterogeneous cell populations such as whole BM, low-density mononuclear and mesenchymal stem (MSCs), and several immunomagnetically separated cell populations were investigated. Among them, MSCs and CD90(+) cells were demonstrated to express neuro-glial transcripts before any treatment. Several culture conditions with the addition of stem cell or astroblast conditioned media, different concentrations of serum, growth factors, and supplements, used alone or in combinations, were demonstrated to alter the cellular morphology in some cell subpopulations. In particular, MSCs and CD90(+) cells acquired astrocytic and neuron-like morphologies in specific culture conditions. They expressed several neuro-glial specific markers by RT-PCR and glial fibrillary acid protein by immunocytochemistry after co-culture with astroblasts, both in the absence or presence of cell contact. In addition, floating neurosphere-like clones have been observed when CD90(+) cells were grown in neural specific media. In conclusion, among the large variety of human adult BM cell populations analyzed, we demonstrated the in vitro neuro-glial potential of both the MSC and CD90(+) subset of cells. Moreover, unidentified soluble factors provided by the conditioned media and Cellular contacts in co-culture systems were effective in inducing the neuro-glial phenotype, further supporting the adult BM neural differentiative capability. (c) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:312 / 325
页数:14
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