Investigation of metabolism using TLC-DAR and reaction-displacement TLC

被引：10

作者：

Kalász, H

Lengyel, J

Szarvas, T

Morovján, G

Klebovich, I

机构：

[1] Semmelweis Univ, Dept Pharmacol & Pharmacotherapy, H-1089 Budapest, Hungary

[2] Semmelweis Univ, Cent Isotope Lab, H-1089 Budapest, Hungary

[3] EGIS Pharmaceut Co Ltd, Dept Pharmacokinet, H-1106 Budapest, Hungary

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2003年 / 16卷 / 05期

关键词：

thin-layer chromatography-digital autoradlography; formaldehyde; formaldemedone; metabolism; N-demethylation; (-)-deprenyl;

D O I：

10.1556/JPC.16.2003.5.10

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Thin-layer chromatography has been used to monitor formaldehyde production during in-vivo metabolism of (-)-deprenyl. Formaldehyde was reacted with dimedone to yield its dimedone adduct, formaldemedone. Determination of C-14-labeled formaldemedone was performed by planar displacement chromatography. The separated bands were detected either by digital autoradiography or on X-ray film after contact autoradiography. Two-dimensional separations have improved the evaluation.

引用

页码：381 / 385

页数：5

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