Safe and effective regulation of hematocrit by gene gun administration of an erythropoietin-encoding DNA plasmid

被引:22
作者
Klinman, DM
Conover, J
Leiden, JM
Rosenberg, AS
Sechler, JMG
机构
[1] US FDA, Retroviral Immunol Sect, Div Viral Prod, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA
[2] Univ Chicago, Dept Med, Chicago, IL 60637 USA
[3] US FDA, Immunol Lab, Div Hematol Prod, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA
关键词
D O I
10.1089/10430349950018733
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This work examines the effect of delivering a DNA plasmid encoding murine erythropoietin (pVRmEpo) to BALB/c mice by gene gun, Whereas intramuscular injection elicits a rise in hematocrit persisting >8 months, intradermal delivery triggers the dose-dependent secretion of biologically active erythropoietin (Epo) for similar to 1 month. Repeated administration of pVRmEpo by gene gun elicits a stable increase in hematocrit. The source of the Epo produced following gene gun delivery was analyzed by periodically grafting the site of injection onto naive recipients. Results indicate that both stationary cells (presumably keratinocytes) and migratory (presumably dendritic) cells were transfected and secreted biologically active Epo in vivo. Gene gun administration of plasmid DNA appears to be safe, and provides an additional strategy for achieving the regulated secretion of an exogenous gene product.
引用
收藏
页码:659 / 665
页数:7
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