PrimeArray: genome-scale primer design for DNA-microarray construction

被引:40
作者
Raddatz, G
Dehio, M
Meyer, TF
Dehio, C
机构
[1] Max Planck Inst Biol, Infekt Biol Abt, D-72076 Tubingen, Germany
[2] Max Planck Inst Infekt Biol, Mol Biol Abt, D-10117 Berlin, Germany
[3] Univ Basel, Bioctr, Abt Mol Mikrobiol, CH-4056 Basel, Switzerland
关键词
D O I
10.1093/bioinformatics/17.1.98
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
PrimeArray is a Windows program that computes oligonuceotide primer pairs for genome-scale gene amplification by the Polymerase Chain Reaction (PCR). The program supports the automated extraction of coding sequences (CDS) from various input-file formats and allows highly automated primer pair-optimization.
引用
收藏
页码:98 / 99
页数:2
相关论文
共 7 条
[1]   Making and reading microarrays [J].
Cheung, VG ;
Morley, M ;
Aguilar, F ;
Massimi, A ;
Kucherlapati, R ;
Childs, G .
NATURE GENETICS, 1999, 21 (Suppl 1) :15-19
[2]   Primer design for large scale sequencing [J].
Haas, S ;
Vingron, M ;
Poustka, A ;
Wiemann, S .
NUCLEIC ACIDS RESEARCH, 1998, 26 (12) :3006-3012
[3]   PRIMO: A primer design program that applies base quality statistics for automated large-scale DNA sequencing [J].
Li, P ;
Kupfer, KC ;
Davies, CJ ;
Burbee, D ;
Evans, GA ;
Garner, HR .
GENOMICS, 1997, 40 (03) :476-485
[4]   High density synthetic oligonucleotide arrays [J].
Lipshutz, RJ ;
Fodor, SPA ;
Gingeras, TR ;
Lockhart, DJ .
NATURE GENETICS, 1999, 21 (Suppl 1) :20-24
[5]  
Proutski V, 1996, COMPUT APPL BIOSCI, V12, P253
[6]  
Suggs S. V., 1981, ICN-UCLA Symp. Developmental Biology, V23, P683
[7]   Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes [J].
Sugimoto, N ;
Nakano, S ;
Yoneyama, M ;
Honda, K .
NUCLEIC ACIDS RESEARCH, 1996, 24 (22) :4501-4505