Trapping of proteins under physiological conditions in a nanopipette

被引：96

作者：

Clarke, RW ^{[1
]}

White, SS ^{[1
]}

Zhou, DJ ^{[1
]}

Ying, LM ^{[1
]}

Klenerman, D ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 24期

基金：

英国生物技术与生命科学研究理事会;

关键词：

biomolecule trapping; DNA; electrophoresis; nanotechnology; proteins;

D O I：

10.1002/anie.200500196

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Caught in a trap and on camera: A nanopipette is used for electrodeless dielectrophoresis and clear evidence is shown, by using wide-field fluorescence imaging (see image), for the reversible trapping of Alexa-488-labeled proteins (protein G and immunoglobulin G) and also of the fluorophore alone. The dielectrophoretic concentration is enhanced by at least a factor of 300 for these fluorophore-labeled proteins. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：3747 / 3750

页数：4

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