Inactivation of Staphylococcus aureus in Water by a Cold, He/O2 Atmospheric Pressure Plasma Microjet

被引：89

作者：

Bai, Na ^{[4
,5
]}

Sun, Peng ^{[3
]}

Zhou, Haixia ^{[4
,5
]}

Wu, Haiyan ^{[3
]}

Wang, Ruixue ^{[5
]}

Liu, Fuxiang ^{[4
,5
]}

Zhu, Weidong ^{[1
,2
]}

Lopez, Jose L. ^{[1
,2
]}

Zhang, Jue ^{[3
,5
]}

Fang, Jing ^{[3
,5
]}

机构：

[1] St Peters Coll, Dept Appl Sci & Technol, Jersey City, NJ USA

[2] St Peters Coll, Ctr Microplasma Sci & Technol, Jersey City, NJ USA

[3] Peking Univ, Coll Engn, Beijing 100871, Peoples R China

[4] Sichuan Univ, W China Coll Stomatol, Chengdu 610064, Peoples R China

[5] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

来源：

PLASMA PROCESSES AND POLYMERS | 2011年 / 8卷 / 05期

关键词：

atmospheric pressure; inactivation; oxygen species; plasma microjet; HYDROGEN-PEROXIDE; AIR PLASMA; ATOMIC OXYGEN; ENVIRONMENT; RADICALS;

D O I：

10.1002/ppap.201000078

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

A direct-current, atmospheric pressure, cold plasma microjet (PMJ) sustained in a quasi-steady gas cavity in liquid was used to inactivate Staphylococcus aureus suspended in distilled water. While helium gas (with 2% O-2 as additive) was used as working gas, an effective inactivation(>99%) was achieved in 6 min. The inactivation of bacteria was further verified by surface morphology examination and LIVE/DEAD Baclight bacterial viability test (fluorescence microscopy). The overall pH and temperature of the liquid were monitored during the plasma treatment and were found to be below the critical values for the survival of S. aureus. Hydroxyl radical ((OH)-O-center dot) was detected via electron spin resonance (ESR) spectroscopy, and alongside other intermediate reactive species, is attributed to the effective inactivation of S. aureus. End-on optical emission spectroscopy show strong atomic oxygen emission both in air and in water.

引用

页码：424 / 431

页数：8

共 34 条

[1]

AIKENS J, 1991, J BIOL CHEM, V266, P15091

[2] Against all odds: The survival strategies of Deinococcus radiodurans [J].

Battista, JR .

ANNUAL REVIEW OF MICROBIOLOGY, 1997, 51 :203-224

[3]

BIELSKI BHJ, 1983, J BIOL CHEM, V258, P4759

[4] Electronic quenching of OH(A) by water in atmospheric pressure plasmas and its influence on the gas temperature determination by OH(A-X) emission [J].

Bruggeman, Peter ;

Iza, Felipe ;

Guns, Peter ;

Lauwers, Daniel ;

Kong, Michael G. ;

Gonzalvo, Yolanda Aranda ;

Leys, Christophe ;

Schram, Daan C. .

PLASMA SOURCES SCIENCE & TECHNOLOGY, 2010, 19 (01)

[5] Non-thermal plasmas in and in contact with liquids [J].

Bruggeman, Peter ;

Leys, Christophe .

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 2009, 42 (05)

[6] VIABLE BUT NON-CULTURABLE VIBRIO-CHOLERAE AND RELATED PATHOGENS IN THE ENVIRONMENT - IMPLICATIONS FOR RELEASE OF GENETICALLY ENGINEERED MICROORGANISMS [J].

COLWELL, RR ;

BRAYTON, PR ;

GRIMES, DJ ;

ROSZAK, DB ;

HUQ, SA ;

PALMER, LM .

BIO-TECHNOLOGY, 1985, 3 (09) :817-820

[7] The Interaction of a Direct-Current Cold Atmospheric-Pressure Air Plasma With Bacteria [J].

Feng, Hongqing ;

Sun, Peng ;

Chai, Yufeng ;

Tong, Guohua ;

Zhang, Jue ;

Zhu, Weidong ;

Fang, Jing .

IEEE TRANSACTIONS ON PLASMA SCIENCE, 2009, 37 (01) :121-127

[8] SUPEROXIDE RADICAL AND SUPEROXIDE DISMUTASES [J].

FRIDOVICH, I .

ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :97-112

[9]

GLAZE WH, 1987, OZONE-SCI ENG, V9, P335

[10] OXYGEN-TOXICITY, OXYGEN RADICALS, TRANSITION-METALS AND DISEASE [J].

HALLIWELL, B ;

GUTTERIDGE, JMC .

BIOCHEMICAL JOURNAL, 1984, 219 (01) :1-14

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