Two-dimensional electrophoresis database of fluorescence-labeled proteins of colon cancer cells

被引：10

作者：

Mori, Y

Kondo, T

Yamada, T

Tsuchida, A

Aoki, T

Hirohashi, S

机构：

[1] Natl Canc Ctr, Inst Res, Canc Proteom Project, Chuo Ku, Tokyo 1040045, Japan

[2] Tokyo Med Univ, Dept Surg 3, Tokyo, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 823卷 / 02期

关键词：

proteome; two-dimensional gel electrophoresis; 2D database; 2D-DIGE; colon cancer;

D O I：

10.1016/j.jchromb.2005.05.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a novel database of the proteome of DLD-1 colon cancer cells by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of fluorescence-labeled proteins followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis. The database consists of 258 functionally categorized proteins corresponding to 314 protein spots. The majority of the proteins are oxidoreductases, cytoskeletal proteins and nucleic acid binding proteins. Phosphatase treatment showed that 28% of the protein spots on the gel are phosphorylated, and mass spectrometric analysis identified 21 of them. Proteins of DLD-1 cells and of laser-microdissected colon cancer tissues showed similar distribution on 2D gels, suggesting the utility of our database for clinical proteomics. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：82 / 97

页数：16

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